Transcriptional activation domain of p300/CBP-associated factor(P/CAF)

p300/CBP相关因子(P/CAF)转录激活域

基本信息

批准号：
09672226
负责人：
NISHIKAWA Jun-ichi
金额：
$ 1.73万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

In eukaryores, transcription of mRNA-encoding classII genes involves the ordered recruitment of general factors and the RNA polymerase II holoenzyme into the basal transcription pre-initiation complex on promoter.The transcription is efficiently enhanced by transcriptional activators which bind to specific DNA sequences outside of the core promoter.Sequence-specific activators work in conjunction with other factors which do not bind DNA directly.These factors are termed co-activators and are recruited to promoters via their interaction with the DNA-bound transcription factors.P/CAF was originally identified as a p300/CBP-binding protein by virtue of its sequence similarity to a yeast histone acetyltransferase (HAT), namely yGCN5.In addition, P/CAF interacts with other co-activators such as ACIR and SRC-1 which are also HAT.In this study, I focused on the function of P/CAF in the transcriptional regulation and investigated the transcriptional activation domain of P/CAF.In order to examine the activation function of P/CAF, I expressed various regions of P/CAF as GALA DNA binding domain in yeast.P/CAF indeed worked as a transcriptional activator protein in yeast and the region of amino acids 366-654 was necessary for activation function.As this region is identical to the HAT domain, the activation function of P/CAF might be related to the acetylation of histones.On the other hand, the interaction of P/CAF with CBP was independent on the activation domain. The complex formation of co-activators in vitro was also examined using surface plasmon resonance anaysis.

在真实性中，mRNA编码的分类基因的转录涉及募集一般因素和RNA聚合酶II全酶在启动子上的基础转录预启用复合物中的均可有效地增强。转录通过与核心启动子外部结合的特定DNA序列的转录激活剂有效地增强。与核心启动因子的连接量直接启动。 are termed co-activators and are recruited to promoters via their interaction with the DNA-bound transcription factors.P/CAF was originally identified as a p300/CBP-binding protein by virtue of its sequence similarity to a yeast histone acetyltransferase (HAT), namely yGCN5.In addition, P/CAF interacts with other co-activators such as ACIR and SRC-1 which are also HAT.In这项研究，我关注P/CAF在转录调控中的功能，并研究了P/CAF的转录激活结构域，以检查P/CAF的激活功能，我表达了P/CAF的各个区域为GALA DNA结合结构域，作为Yeast.p/Caf中的Yeast.p/Caf中的p/caf的各个区域，实际上是在Yeast蛋白中工作的366-66-66-6-66-6-66-6-66-66-16-6666.与HAT结构域相同，P/CAF的激活函数可能与组蛋白的乙酰化有关。另一方面，P/CAF与CBP的相互作用在激活域独立。还使用表面等离子体共振ANAYSIS检查了体外共激活剂的复杂形成。