Selective secretion mechanism of Wnt in polarized epithelial cells

极化上皮细胞Wnt的选择性分泌机制

• 批准号: 24790280
• 负责人: SAKANE Hiroshi
• 金额: $ 2.83万
• 依托单位: Dokkyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24790280/
• 关键词: 細胞内シグナル伝達; Wnt; リサイクリング; 上皮細胞; 選別輸送; クラスリン

Selective basolateral secretion of Wnt3a in polarized epithelial cells was regulated by Wntless, clathrin and clathrin adaptor proteins. We found that Wntless was localized to the basolateral membrane. We also found that Wnt11 was sorted to the apical side and glycosylation was involved in the selective secretion of Wnt proteins. Furthermore, we found that a-taxilin, a syntaxin4 binding protein, was involved in the recycling of transferrin receptor. These results clarified a part of mechanism of selective secretion of Wnt in polarized epithelial cells and the function of regulator of intracellular vesicle traffic.

极化上皮细胞中 Wnt3a 的选择性基底外侧分泌受到 Wntless、网格蛋白和网格蛋白接头蛋白的调节。我们发现 Wntless 位于基底外侧膜。我们还发现Wnt11被分选到顶端侧并且糖基化参与Wnt蛋白的选择性分泌。此外，我们发现α-taxilin（一种突触蛋白4结合蛋白）参与转铁蛋白受体的再循环。这些结果阐明了极化上皮细胞选择性分泌Wnt的部分机制以及细胞内囊泡运输的调节功能。

项目成果

α-Taxilin interacts with soring nexin 4 and participates in the recycling pathway of transferrin receptor.

α-Taxilin 与 soring nexin 4 相互作用并参与转铁蛋白受体的循环途径。

• DOI: 10.1371/journal.pone.0093509
• 发表时间: 2014
• 影响因子: 3.7
• 作者: Sakane; H.; Horii; Y.; Nogami; S.; Kawano; Y.; Kaneko;Shirataki; H.
• 通讯作者: H.

The apical and basolateral secretion of Wnt11 and Wnt3a in polarized epithelial cells is regulated by distinct mechanisms.

极化上皮细胞中 Wnt11 和 Wnt3a 的顶端和基底外侧分泌受到不同机制的调节。

• DOI: 10.1242/jcs.126052
• 发表时间: 2013
• 作者: Yamamoto; H.; Awada; C.; Hanaki; H.; Sakane; H.; Tsujimoto; I.; Takahashi; Y.; Takao; T.;Kikuchi; A.
• 通讯作者: A.