DNA Phylogeny of Boreal Conifers from the Last Glacial Age in Japan

日本末次冰期北方针叶树的 DNA 系统发育

• 批准号: 09839004
• 负责人: SUZUKI Mitsuo
• 金额: $ 2.37万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09839004/
• 关键词: fossil; cone; DNA; Last Glacial Age; Honshu; Picea; boreal conifer; 亜塞帯性針葉樹; 積分化

The most parts of Honshu Island were covered by boreal conifer forests during the Last Glacial Age. From sedimentations of those age, there are much amounts of fossil cones with beautiful preservation. But, to identify them in species level only bymorphological characters is very difficult because of close similarity in morphology. Therefore, we tried to extract chioroplast DNA from those fossils and to identify there by mean of DNA sequence. For this purpose1 we collected extant conifers such as Picea bicolor r, P jezoensis, P glehnii, P koyamae, P.maximowiczii, P.palita , and P shirasawae and established phylogenetic tree basing on DNA sequence.Fossil cones were collected from four localities in northern Honshu. Those samples were kept in liquid nitrogen and extracted total DNA, then multiplied by mean of PCR method. We succeed to read DNA sequence from several fossils. DNA of fossils obtained from buried forest in Dekijima, Aomori Prefecture (about 24,000 years ago) was identical with Picea glehnii , while that from buried forest in Gonohe county, Aomori Prefecture (about 13,600 years ago) was identical with Picea koyamae. In both localities there is no distribution of those Picea species. From other two localities, we can not yet success to read DNA.

在最后一个冰川时代，洪斯胡岛的大部分地区被北方针叶树森林覆盖。从这些年龄的沉积物中，有很多化石锥具有美丽的保存。但是，在物种层面上识别它们，只有副形性特征非常困难，因为形态上的相似性非常相似。因此，我们试图从这些化石中提取chioroplast DNA，并通过DNA序列的平均值来识别。为此，我们收集了现有的针叶树，例如Picea Bicolor R，P Jezoensis，P Glehnii，P Koyamae，P.Maximowiczii，P.Palita和P Shirasawae，并在DNA序列上建立了从北部北部的dna se se se se sepence in Northnernnernnernnernities in northnern shonshu收集的系统植物基础。将这些样品保存在液氮中并提取总DNA，然后乘以PCR方法的平均值。我们成功阅读了几种化石的DNA序列。从Dekijima埋葬的森林获得的化石DNA，Aomori县（大约24,000年前）与Picea Glehnii相同，而来自Aomori县的Gonohe县（大约13,600年前）的DNA与Picea Koyamae相同。在这两个地方都没有那些PICEA物种的分布。从其他两个地区，我们还无法成功阅读DNA。

项目成果

Noshiro, S., Terada, K., Tsuji, S. & Suzuki, M.: "Larix-Picea forests of the lastgracialage on the eastern elope of Towada Volcano in northern Japan." Rev. Paleobot. & Palynology. 98. 207-222 (1997)

能代，S.，寺田，K.，辻，S.