Development of recombinant ELISA for diagnosis of Lassa fever in Africa and structural and biochemical characterisation of the replication complex of Lassa virus

开发用于诊断非洲拉沙热的重组 ELISA 以及拉沙病毒复制复合体的结构和生化特征

• 批准号: 68769158
• 负责人: Professor Dr. Stephan Günther
• 金额: --
• 依托单位: Redeemers University
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/68769158?language=en
• 关键词: Development; recombinant; ELISA; diagnosis; Lassa

The project combines applied research that is directly relevant to the management of Lassa fever in the endemic area with basic research on structure and function of the Lassa virus replication complex. Research in both fields is based on cutting-edge technologies and will be jointly conducted by African and German scientists taking advantage of existing long-term collaborations.Scientific objectives are:1. Development of recombinant IgM and IgG ELISA for diagnosis of Lassa fever in the endemic area.2. Epidemiological studies as well as isolation and full-length sequencing of Lassa virus strains circulating in Nigeria and use of the data for comprehensive bioinformatic analysis.3. Characterisation of Lassa virus L protein using the replicon system.4. Identification and characterisation of enzymatic functions associated with the L protein using heterologous expression systems for full-length L protein as well as individual domains.5. Structural and biochemical characterisation of individual domains of Lassa virus L protein, Z protein, and nucleoprotein (NP).6. Long-term objective: Structure/function guided design and synthesis of compounds to inhibit enzymatic functions or interactions of L protein.

该项目结合了与地方性区域LASSA热管理直接相关的应用研究，以及关于LASSA病毒复制复合复合综合体的结构和功能的基础研究。这两个领域的研究均基于最先进的技术，并将由非洲和德国科学家共同进行现有的长期合作。Scientific目标是：1。重组IgM和IgG ELISA的开发用于诊断流行区域的LASSA热。2。尼日利亚循环的LASSA病毒菌株的流行病学研究以及分离和全长测序以及用于全面生物信息学分析的数据。3。使用复制子系统对LASSA病毒L蛋白的表征4。使用异源表达系统与L蛋白相关的酶促功能的鉴定和表征，用于全长L蛋白以及单个结构域。5。 LASSA病毒L蛋白，Z蛋白和核蛋白（NP）单个结构域的结构和生化表征。6。长期目标：结构/功能引导的设计和化合物的合成以抑制L蛋白的酶促功能或相互作用。