Identification and functional characterization of proteins associated with membrane fractions surrounding activating and inhibitory Natural Killer cell receptors

围绕激活和抑制自然杀伤细胞受体的膜组分相关蛋白的鉴定和功能表征

• 批准号: 68359804
• 负责人: Professor Dr. Carsten Watzl
• 金额: --
• 依托单位: Leibniz-Institut für Arbeitsforschung an der TU Dortmund (IfADo)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2008-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/68359804?language=en
• 关键词: Identification; functional; characterization; proteins; associated

Natural Killer (NK) cells play an important role in the immune response against viral infections and cancer. The function of NK cells is regulated by a balance of positive and negative signals transmitted by activating or inhibitory cell surface receptors. The membrane domains surrounding these receptors play an integral role in the regulation of NK cell activity. While activating receptors need to associate with specialized membrane domains (Detergent resistant membrane fractions and GM1- rich microdomains) in order to activate NK cell functions, inhibitory receptors prevent the association of activating receptors with these domains and thereby control NK cell activity. We will therefore study the distribution of membrane microdomains during NK cell activation and inhibition. By identification and functional characterization of specific protein components of membrane domains surrounding activating and inhibitory receptors in NK cells we aim to get a better understanding of NK cell regulation and to identify novel targets for the manipulation of NK cell activity.

自然杀伤 (NK) 细胞在针对病毒感染和癌症的免疫反应中发挥着重要作用。 NK 细胞的功能通过激活或抑制性细胞表面受体传递的正信号和负信号的平衡来调节。这些受体周围的膜域在 NK 细胞活性的调节中发挥着不可或缺的作用。虽然激活受体需要与专门的膜结构域（耐洗涤剂的膜组分和富含 GM1 的微结构域）结合才能激活 NK 细胞功能，但抑制性受体阻止激活受体与这些结构域结合，从而控制 NK 细胞活性。因此，我们将研究 NK 细胞激活和抑制过程中膜微区的分布。通过对 NK 细胞激活和抑制受体周围膜域的特定蛋白质成分进行鉴定和功能表征，我们的目标是更好地了解 NK 细胞调节，并确定操纵 NK 细胞活性的新靶点。