AGE-RELATED CHANGES IN THE CARDIAC RESPONSE TO ENDOTHELIN-1 AND THE RECEPTOR IN RAT VENTRICULAR MYOCARDIUM.
大鼠心室心肌内皮素-1 和受体的心脏反应与年龄相关的变化。
基本信息
- 批准号:08838001
- 负责人:
- 金额:$ 1.41万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1996
- 资助国家:日本
- 起止时间:1996 至 1997
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Endothelin (ET) is an endogenous potent vasoconstrictor, which may be involved in the pathogenesis of various cardiovascular diseases. It has been known that a high density of ET receptors is found in mammalian myodardium and myocardial ET receptors are up-regulated in some pathologic states. We have found that ET-1-induced coronary vasoconstriction increased with age and myocardial ET receptors are up-regulated in aged rat in compare with young rat.In this study we examined the mechanisms of the age-related changes in the cardiac response to ET-1 and gene expression of ET^A-and ET^B-receptors as well as age-related changes in the binding characteristics of ET receptor in rat ventricular myocardium.Results : In experiments with isolated papillary muscles, ET-1 elicited a positive inotropic effect in the young rat, but not in aged rat. A SR-Ca-ATP ase inhibitor, cyclopiazonic acid, concentration-dependently inhibited the positive inotropic effects in the young rat. Receptor assay showed that the affinity of ET-receptor was not age-related differences, but the density (Bmax) of ET-binding was significantly increased in aged rats. Gene expression of both ET^A-and ET^B-receptors determined by Northern blotting analysis or semiquantitative RT-PCR increased in aged rats, but not significantly. The ratio of ET^A-and ET^B-receptors mRNA was not changed, suggesting that the alteration of responsiveness to ET is not due to the change in the ratio of ET^A-and ET^B-receptors. These findings suggest that the decrease in the function of SR Ca-ATP ase may be responsible for the marked attenuation of the ET-induced positive inotropic effects in the aged rat. Alternatively the increase in ET-receptor in aged rats may be an adaptation to decreased cardiac responsiveness to ET-1.
内皮素(ET)是一种内源性有效的血管收缩剂,可能与各种心血管疾病的发病机理有关。众所周知,在哺乳动物肌心肌中发现了高密度的ET受体,在某些病理状态下,心肌ET受体被上调。 We have found that ET-1-induced coronary vasoconstriction increased with age and myocardial ET receptors are up-regulated in aged rat in compare with young rat.In this study we examined the mechanisms of the age-related changes in the cardiac response to ET-1 and gene expression of ET^A-and ET^B-receptors as well as age-related changes in the binding characteristics of ET receptor in rat ventricular myocardium.Results : In分离的乳头肌肉的实验ET-1引起了年轻大鼠的阳性肌力作用,但在老年大鼠中没有引起阳性。 SR-CA-ATP ASE抑制剂环皮二唑酸浓度依赖性地抑制了年轻大鼠的阳性肌力作用。受体分析表明,ET受体的亲和力不是与年龄相关的差异,但是老年大鼠的ET结合的密度(BMAX)显着增加。通过北印迹分析或半定量RT-PCR确定的ET^a和et^b受体的基因表达在老年大鼠中增加,但没有显着。 Et^a-and et^b受体mRNA的比率没有改变,这表明对ET的反应性的改变不是由于Et^a-and et^b受体比率的变化所致。这些发现表明,SR CA-ATP ASE功能的下降可能是造成ET诱导的老年大鼠阳性正性效应的明显衰减的原因。或者,老年大鼠ET受体的增加可能是对降低对ET-1的心脏反应性的适应性。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Akira Ishihata: "Aging-associated adaptation of the rat heart by increased expression of the endothelin-receptors." Cardiac Structure and Metabolism. 20(in press). (1998)
Akira Ishihata:“通过增加内皮素受体的表达来实现与衰老相关的大鼠心脏的适应。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Zhe-Long XU: "Effect of ischemic preconditioning on myocardial oxygen consumption durihg ischemi a." J.Mol.Cell Cadiol.(印刷中). (1998)
徐哲龙:“缺血预处理对缺血期间心肌耗氧量的影响。”(J.Mol.Cell Cadiol)(出版中)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Akira Ishihata: "Inhibition of the expression of the gene for the angiotensin ATl receptor by angiotensin II in the rat adredel gland." European J. Pharmacol. (印刷中). (1998)
Akira Ishihata:“血管紧张素 II 在大鼠 adredel 腺中抑制血管紧张素 AT1 受体基因的表达”,European J. Pharmacol(出版中)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Yumi Katano: "Age-related changes in the contractile response to endothelin-1 and in binding characteristics of the endothelin receptor in rat ventricular myocardium." The Developing Heart.301-310 (1997)
Yumi Katano:“大鼠心室心肌内皮素-1 收缩反应和内皮素受体结合特征与年龄相关的变化。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Zhe-Long Xu: "Effect of ischemic preconditioning on myocardial oxygen consumpting during ischemia." J,Mol,Cell,Cardiol.(印刷中). (1998)
徐哲龙:“缺血预处理对缺血期间心肌耗氧量的影响”,J,Mol,Cell,Cardiol。(出版中)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
KATANO Yumi其他文献
KATANO Yumi的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('KATANO Yumi', 18)}}的其他基金
The importance of hypertriglyceridemia in progressof atherosclerosis
高甘油三酯血症在动脉粥样硬化进展中的重要性
- 批准号:
19590239 - 财政年份:2007
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Mechanism of impairment of NO production by aging and trials for the amelioration of endothelial function
衰老导致 NO 产生受损的机制以及改善内皮功能的试验
- 批准号:
15590220 - 财政年份:2003
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
THE MECHANISM OF AGE-RELATED CHANGES IN THE RELEASE OF NITRIC OXIDE.
一氧化氮释放与年龄相关的变化机制。
- 批准号:
11670658 - 财政年份:1999
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysis of aging-associated genes in myocardium : application of RT-PCR differential display method.
心肌衰老相关基因分析:RT-PCR差异显示法的应用。
- 批准号:
09557008 - 财政年份:1997
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Functional relevance of the changes in cyclic AMP accumulation.
环 AMP 积累变化的功能相关性。
- 批准号:
03670090 - 财政年份:1991
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
相似国自然基金
内皮素B型受体信号通路在少突胶质细胞分化以及在脱髓鞘疾病治疗中的应用研究
- 批准号:
- 批准年份:2022
- 资助金额:54 万元
- 项目类别:面上项目
肺血管结构重构调控的新机制:内源性硫化氢介导的内皮素A型受体过硫化修饰
- 批准号:82270428
- 批准年份:2022
- 资助金额:52.00 万元
- 项目类别:面上项目
内皮素受体EDNRB导致耳聋、听力损失的分子机制及药理学研究
- 批准号:82271190
- 批准年份:2022
- 资助金额:52.00 万元
- 项目类别:面上项目
内皮素B型受体信号通路在少突胶质细胞分化以及在脱髓鞘疾病治疗中的应用研究
- 批准号:32270851
- 批准年份:2022
- 资助金额:54.00 万元
- 项目类别:面上项目
肺血管结构重构调控的新机制:内源性硫化氢介导的内皮素A型受体过硫化修饰
- 批准号:
- 批准年份:2022
- 资助金额:52 万元
- 项目类别:面上项目
相似海外基金
Salt Mediated Cross Talk Between Lymphatic Vessels and Immune Cells in Kidney Disease
盐介导肾脏疾病中淋巴管和免疫细胞之间的交互作用
- 批准号:
10636755 - 财政年份:2023
- 资助金额:
$ 1.41万 - 项目类别:
Identifying Novel Signaling Mechanisms Downstream of Cardiac Gq-Coupled Receptors
鉴定心脏 Gq 偶联受体下游的新型信号传导机制
- 批准号:
10535591 - 财政年份:2023
- 资助金额:
$ 1.41万 - 项目类别:
Crosstalk Ca2+ Signaling between Ryanodine Receptors Type 1 and 2 in the Pathogenesis of Cardiac Hypertrophy and Heart Failure
心脏肥大和心力衰竭发病机制中 1 型和 2 型 Ryanodine 受体之间的串扰 Ca2 信号传导
- 批准号:
10660636 - 财政年份:2023
- 资助金额:
$ 1.41万 - 项目类别:
Pulmonary Vascular Development in Single Ventricle Heart Disease: A Longitudinal Biomarker Approach
单心室心脏病的肺血管发育:纵向生物标志物方法
- 批准号:
10591167 - 财政年份:2023
- 资助金额:
$ 1.41万 - 项目类别: