Study on the molecules responsible for implantation window

负责植入窗口的分子的研究

• 批准号: 23659767
• 负责人: SUZUKI Hiroshi
• 金额: $ 2.33万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659767/
• 关键词: 着床; 胚; 遺伝子; 妊娠; 偽妊娠

To develop a control method for embryonic implantation and subsequent differentiation and proliferation as well as recognition of embryonic attachment in uterus, expression pattern of genes responsible for initiation of implantation window in uterine tissues from an identical recipient which transferred fertilized oocytes at pronucleus stage and embryos at 8-cell stage into right and left side of the oviduct, respectively, was examined by using a DNA microarray. As a result, at the open of implantation window, one down-regulated and two up-regulated genes were identified. These presumably responsible genes for window of implantation were further analyzed the expression profile during the course of preimplantation development and implantation by a real time PCR method. Expression of Sprr2f was highest on 0.5 dpc and gradually decreased with preimplantation development and during implantation. However, Sprr2f strongly expressed in ovarian tissues on 3.5 dpc. Ass1 highly expressed in uterus on 4.5 dpc and in ovary on 3.5 dpc. Further analysis of these molecules as candidates of genes responsible for regulation of implantation window is required in uterine and ovarian tissues at peri-implantation period.

开发一种控制方法，用于胚胎着床和随后的分化和增殖，以及识别子宫中的胚胎附着，负责在原核阶段转移受精卵母细胞和在原核阶段转移胚胎的相同受体的子宫组织中负责着床窗口启动的基因的表达模式。使用 DNA 微阵列分别检查输卵管右侧和左侧的 8 细胞阶段。结果，在植入窗口打开时，鉴定出一个下调基因和两个上调基因。通过实时PCR方法进一步分析了这些可能与植入窗口相关的基因在植入前发育和植入过程中的表达谱。 Sprr2f 的表达在 0.5 dpc 时最高，并随着植入前发育和植入过程逐渐下降。然而，Sprr2f 在 3.5 dpc 时在卵巢组织中强烈表达。 Ass1 在 4.5 dpc 时在子宫中高表达，在 3.5 dpc 时在卵巢中高表达。需要在围着床期的子宫和卵巢组织中对这些分子作为负责调节着床窗口的候选基因进行进一步分析。

项目成果

Beneficial effect of hyperbaric oxygen therapy on the follicular survival in the mouse ovary after transplantation

高压氧治疗对小鼠卵巢移植后卵泡存活的有益作用

• 发表时间: 2012
• 作者: Hayashi; S.; Asano; T.; Kakizaki; R.; Suzuki; H.
• 通讯作者: H.

Effect of intraperitoneal administration of desialylated erythropoietin on the follicular survival in cryopreserved canine ovaries after xenotransplantation

腹腔注射去唾液酸促红细胞生成素对犬卵巢异种移植后卵泡存活的影响

• 发表时间: 2011
• 作者: Hirayama; Y.; Inoue; K.; Suzuki; H.
• 通讯作者: H.

Cryopreservation of canine embryos and resulting pregnancies.

犬胚胎的冷冻保存和由此产生的妊娠。

• 发表时间: 2012
• 作者: Suzuki; H.
• 通讯作者: H.

Sexing of dog sperm by fluorescene in situ hybridization.

通过荧光原位杂交对狗精子进行性别鉴定。

• 发表时间: 2012
• 作者: Oi; M.; Yamada; K.; Hayakawa; H.;Suzuki; H.
• 通讯作者: H.

マラリア原虫感染が妊娠時特異的に重篤化する免疫学的メカニズムの解明

阐明妊娠期间疟原虫感染变得特别严重的免疫机制

• 发表时间: 2012
• 作者: 新田あかね;白砂孔明;松本茜;鈴木宏志
• 通讯作者: 鈴木宏志