Mechanism of intracellular persistence of Staphylococcus aureus by novel function of toxic shock syndrome toxin-1

中毒性休克综合征毒素-1的新功能对金黄色葡萄球菌细胞内持久性的机制

• 批准号: 23790467
• 负责人: ASANO Krisana
• 金额: $ 2.75万
• 依托单位: Hirosaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790467/
• 关键词: 黄色ブドウ球菌; TSST-1; オートファジー; 感染症; 細胞内寄生

Effect of toxic shock syndrome toxin-1 (TSST-1) on Staphylococcus aureus infection in the epithelial cells was investigated. TSST-1 did not alter the adhesion and invasion into the epithelial cells, HeLa 229, HEK293 and 407. However, TSST-1 reduced the accumulation of autophagosomes in nutrient starved cells or rapamycin-treated cells, suggesting that TSST-1 suppresses autophagy. In addition, this suppression did not require superantigenic activity and might be involved in the autophagosome formation process rather than autophagosome degradation. From day 5 after infection, intracellular bacterial number and cytotoxicity of TSST-1-producing S. aureus were deceased in comparison to non-producing strain, suggesting that TSST-1 may promote intracellular persistence of S. aureus in the epithelial cells.

研究了中毒性休克综合征毒素-1 (TSST-1) 对上皮细胞金黄色葡萄球菌感染的影响。 TSST-1 不会改变对上皮细胞、HeLa 229、HEK293 和 407 的粘附和侵袭。然而，TSST-1 减少了营养饥饿细胞或雷帕霉素处理细胞中自噬体的积累，表明 TSST-1 抑制自噬。此外，这种抑制不需要超抗原活性，并且可能涉及自噬体形成过程而不是自噬体降解。从感染后第5天开始，与不产生TSST-1的菌株相比，产生TSST-1的金黄色葡萄球菌的细胞内细菌数量和细胞毒性下降，表明TSST-1可能促进金黄色葡萄球菌在上皮细胞中的细胞内持久存在。

项目成果

Interaction of Listeria monocytogenes autolysin amidase with glycosaminoglycans promotes listerial adhesion to mouse hepatocytes

单核细胞增生李斯特菌自溶素酰胺酶与糖胺聚糖的相互作用促进李斯特菌对小鼠肝细胞的粘附

• DOI: 10.1016/j.biochi.2012.02.026
• 发表时间: 2012
• 影响因子: 3.9
• 作者: Asano; K.; Kakizaki; I.;Nakane; A.
• 通讯作者: A.

Inhibition of emetic and superantigenic activities of staphylococcal enterotoxin A by synthetic peptides

合成肽抑制葡萄球菌肠毒素 A 的催吐和超抗原活性

• DOI: 10.1016/j.peptides.2012.08.013
• 发表时间: 2012
• 作者: Edward Kithela Maina

A novel function of Toxic Shock Syndrome Toxin-1 insuppression of autophagy

中毒性休克综合征Toxin-1抑制自噬的新功能

• 发表时间: 2013
• 作者: Asano K;Nakane A
• 通讯作者: Nakane A

Autophagic suppressing activity of staphylococcalToxic Shock Syndrome Toxin-1

葡萄球菌中毒性休克综合征Toxin-1的自噬抑制活性

• 发表时间: 2013
• 作者: Asano K;Nakane A
• 通讯作者: Nakane A

Salmon proteoglycan suppresses progression of mouse experimental autoimmune encephalomyelitis via regulation of Th17 and Foxp3(+) regulatory T cells

鲑鱼蛋白多糖通过调节 Th17 和 Foxp3( ) 调节性 T 细胞抑制小鼠实验性自身免疫性脑脊髓炎的进展

• DOI: 10.1016/j.lfs.2012.09.022
• 发表时间: 2012
• 影响因子: 6.1
• 作者: Sashinami H; Asano K; Yoshimura S; Mori F; Wakabayashi K; Nakane A
• 通讯作者: Nakane A