Monitoring of neuroinflammation and creation of new strategy after spinal cord injury.

脊髓损伤后神经炎症的监测和新策略的制定。

• 批准号: 23791665
• 负责人: SATO Atsushi
• 金额: $ 2.58万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791665/
• 关键词: 脊髄損傷; 神経炎症; マイクログリア; マクロファージ; インターロイキン１; 骨髄間葉系幹細胞; 代替経路型活性化型; 古典的活性化型; ヒト骨髄間葉系幹細胞; PACAP; IL-1; 炎症応答

Microglia and macrophages (MG/MF) have a diverse range of functions depending on unique cytokine stimuli, and contribute to neural cell death, repair, and remodeling during central nervous system diseases. While IL-1 has been shown to exacerbate inflammation, it has also been recognized to enhance neurodegeneration. We determined the activating phenotype of MG/MF and the impact of IL-1 in an in vivo spinal cord injury (SCI) model of IL-1 knock-out (KO) mice. Moreover, we demonstrated the contribution of IL-1 to both the classical and alternative activation of MG in vitro using an adult MG primary culture.We demonstrate here in in vivo experiments that IL-1 suppressed SCI in a process mediated by the reduction of inflammatory responses. Moreover, we suggest that IL-1 participates in both the classical and alternative activation of MG in in vivo and in vitro systems. hMSCs Implanted to injured spinal cord have been recognized to improve motor function, neuroinflammation and regeneration.

小胶质细胞和巨噬细胞 (MG/MF) 根据独特的细胞因子刺激而具有多种功能，并有助于中枢神经系统疾病期间神经细胞的死亡、修复和重塑。虽然 IL-1 已被证明会加剧炎症，但它也被认为会加剧神经退行性变。我们确定了 MG/MF 的激活表型以及 IL-1 在 IL-1 敲除 (KO) 小鼠体内脊髓损伤 (SCI) 模型中的影响。此外，我们使用成人 MG 原代培养物证明了 IL-1 在体外对 MG 经典激活和替代激活的贡献。我们在体内实验中证明，IL-1 在炎症减少介导的过程中抑制 SCI回应。此外，我们认为 IL-1 参与体内和体外系统中 MG 的经典激活和替代激活。植入受损脊髓的 hMSC 已被认为可以改善运动功能、神经炎症和再生。

项目成果

Establishment and characterization of primary adult microglial culture in mice

小鼠原代成年小胶质细胞培养物的建立和表征

• 发表时间: 2013
• 作者: Ohtaki H; Tsumuraya T; Song D; Sato A; Ohara K; Miyamoto K; Nakano H; Kiriyama K; Dohi K; Hiraizumi Y; Matsunaga M; Shioda S
• 通讯作者: Shioda S

Stem cell characteristics and anti-inflammation properties of hMSCs in different passages

不同传代hMSCs的干细胞特性及抗炎特性

• 发表时间: 2013
• 作者: D;an Song; Hirokazu Ohtaki; Tomomi Tsumuraya; Atsushi Sato; Yutaka Hiraizumi;Tomio Inoue; Seiji Shioda
• 通讯作者: Seiji Shioda

脊髄損傷マウスにおけるマイクログリア／マクロファージ活性化へのインターロイキン１の関与

白细胞介素1参与脊髓损伤小鼠小胶质细胞/巨噬细胞的激活

• 发表时间: 2013
• 作者: 佐藤敦;大滝博和;小原賢司;圓谷智海;平泉裕;渥美敬;塩田清二
• 通讯作者: 塩田清二

Human stem/progenitor cells from bone marrow improve spinal cord injury via communicating with PACAP.

来自骨髓的人类干/祖细胞通过与 PACAP 通讯改善脊髓损伤。

• 发表时间: 2013
• 作者: Ohtaki H; Tsumuraya T; Song D; Sato A; Xu Z; Miyamoto K; Hiraizumi Y; Nakamachi T; Hashimoto H; Shintani N; Shioda S
• 通讯作者: Shioda S

Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury

Interleukin-1参与脊髓损伤后小胶质细胞/巨噬细胞的经典和替代激活

• DOI: 10.1186/1742-2094-9-65
• 发表时间: 2012
• 作者: Sato A; Ohtaki H; Tsumuraya T; Song D; Ohara K; Asano M; Iwakura Y; Atsumi T; Shioda S
• 通讯作者: Shioda S