Purification, structure characterization of bioactive substances from marine organisms and application them to the novel targeting oral cancer drugs

海洋生物活性物质的纯化、结构表征及其在口腔癌新型靶向药物中的应用

• 批准号: 23592962
• 负责人: YUKIO YOSHIOKA
• 金额: $ 3.24万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592962/
• 关键词: 海洋生物; 生理活性物質; 抗腫瘍効果; 口腔がん; 癌幹細胞; 分子標的薬; 口腔癌; 口腔癌幹細胞; 分子標的治療薬; がん幹細胞

Marine organisms from Japanese adjacent Sea is collected and freeze-dried. They are homogenized in EtOH-MeOH(4:1)and distributed by HPLC into hexane layer and C2H5OCH3CO layer and aqueous layer. Following it, the extracted compounds are investigated their activity by bio-assay and are purified and decided their structure by LC/MS. Their analogues improving the structure of their branches are produced and massively synthesized them. The anti-tumor activity is investigated by the inoculated tumor cell on nude mouse. CD133 is a useful marker of the oral squamous cell carcinoma cells. The oral cancer stem cells were isolated from the oral squamous cell carcinoma cell line with index of CD133 and analyzed the character of a stem cell. The mechanism of the novel anti-tumor agents against not only the oral cancer but also the oral cancer stem cells is analyzed in vitro.

收集来自日本邻近海域的海洋生物并冷冻干燥。将它们在 EtOH-MeOH(4:1) 中均质化，并通过 HPLC 将其分配到己烷层、C2H5OCH3CO 层和水层中。随后，通过生物测定研究提取的化合物的活性，并通过 LC/MS 纯化并确定其结构。它们的类似物改善了它们的分支结构，并被大量合成。通过裸鼠接种肿瘤细胞来研究其抗肿瘤活性。 CD133 是口腔鳞状细胞癌细胞的有用标记。从标签为CD133的口腔鳞状细胞癌细胞系中分离口腔癌干细胞，并分析干细胞的特性。在体外分析了新型抗肿瘤剂不仅针对口腔癌而且针对口腔癌干细胞的机制。

项目成果

ビスフォスフォネート関連顎骨壊死にて腐骨除去術を行い長期観察し得た骨Paget病の１例

佩吉特骨病一例，其中双磷酸盐相关的颌骨坏死通过牺牲骨去除进行治疗，并且可以进行长期观察。

• 发表时间: 2012
• 作者: 吉岡幸男 虎谷茂昭 向笠英恵 神田拓 新谷智章 岡本哲治

Petrosiols A-E, neurotrophic diyne tetraols isolated from the Okinawan sponge Petrosia strongylata

Petrosiols A-E，从冲绳海绵 Petrosia Strongylata 中分离出的神经营养性二炔四醇

• 发表时间: 2013
• 影响因子: 2.1
• 作者: Kouhei horikawa;Takuya Yagyu; Yukio Yoshioka; Takeshi Fujiwara; Akihiko Kanamoto; Tetsuji Okamoto; Makoto Ojika
• 通讯作者: Makoto Ojika