Exosomal microRNAs as diagnostic biomarkers in metastatic gastric cancer

外泌体 microRNA 作为转移性胃癌的诊断生物标志物

• 批准号: 23590703
• 负责人: OHSHIMA Keiichi
• 金额: $ 3.49万
• 依托单位: Shizuoka Cancer Center Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590703/
• 关键词: エクソソーム; 腫瘍マーカー; microRNA; セクレトーム; プロテオミクス; マイクロアレイ; 培養上清; 質量分析

Exosomes are small vesicles secreted from cells that transport their embedded molecules by a kind of bidirectional exocytosis- and endocytosis-like pathways. Expression patterns of exosomal molecules such as proteins and RNAs can be indicative of cell type since their signature is thought to be unique among cells.Using human cancer cell lines, we have conducted profiling of exosomal microRNAs and proteins using microarray and mass spectrometry. As a result, specific microRNA profiles were found in exosomes derived from scirrhous type gastric and metastatic pancreatic cancer cells, respectively. In addition, a comparative proteome analysis identified exosomal proteins with metastatic marker potential such as polyadenylate binding protein 1 (PABP1) for duodenal cancer and epidermal growth factor receptor pathway substrate (Eps8) for pancreatic cancer.

外泌体是细胞分泌的小囊泡，通过一种双向胞吐作用和内吞作用样途径运输其嵌入的分子。蛋白质和 RNA 等外泌体分子的表达模式可以指示细胞类型，因为它们的特征被认为在细胞中是唯一的。利用人类癌细胞系，我们使用微阵列和质谱法对外泌体 microRNA 和蛋白质进行了分析。结果，分别在源自硬质型胃癌细胞和转移性胰腺癌细胞的外泌体中发现了特定的 microRNA 谱。此外，比较蛋白质组分析确定了具有转移标记潜力的外泌体蛋白，例如十二指肠癌的聚腺苷酸结合蛋白 1 (PABP1) 和胰腺癌的表皮生长因子受体途径底物 (Eps8)。

项目成果

Novel protein isoforms of carcinoembryonic antigen are secreted from pancreatic, gastric and colorectal cancer cells

胰腺癌、胃癌和结直肠癌细胞分泌癌胚抗原的新型蛋白质亚型

• DOI: 10.1186/1756-0500-6-381
• 发表时间: 2013
• 作者: Hatakeyama K; Wakabayashi
• 通讯作者: Wakabayashi

Structure-function relationship of the nuclear localization signal sequence of parathyroid hormone-related protein

甲状旁腺激素相关蛋白核定位信号序列的结构与功能关系

• 发表时间: 2012
• 作者: Ohshima K

膵がん細胞エクソソームに含まれるEps8タンパク質

胰腺癌细胞外泌体中含有的 Eps8 蛋白

• 发表时间: 2013
• 作者: 大島啓一

胃がん患者末梢血注におけるスプライシングバリアント発現レベルの定量化

胃癌患者外周血输注中剪接变异体表达水平的定量

• 发表时间: 2012
• 作者: 畠山慶一

Investigation of proNT/NMN secretion from small cell lung carcinoma cells using a mouse xenograft model

使用小鼠异种移植模型研究小细胞肺癌细胞的 proNT/NMN 分泌

• DOI: 10.3892/or.2012.1926
• 发表时间: 2012
• 作者: Wakabayashi