High-resolution spatiotemporal analysis of harmaline-induced tremor and inferior olive activity in living mice

活体小鼠骆驼蓬碱引起的震颤和下橄榄活动的高分辨率时空分析

• 批准号: 22K06478
• 负责人: ウーシサーリ マルリカ・ヨエ
• 金额: $ 2.58万
• 依托单位: Okinawa Institute of Science and Technology Graduate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K06478/
• 关键词: tremor; harmaline; motion capture; calcium imaging; in vivo; olivo-cerebellar system; essential tremor

In the past year, our dedicated research team has made noteworthy progress in elucidating the kinematic and neuromotor mechanisms of tremor-related disorders. We have successfully initiated a project to determine safe conditions for viral expression of GCamP6 in the inferior olive (IO), which plays a critical role in understanding harmaline-induced tremor in mice. This work's findings will be presented at the esteemed JNS conference in Sendai, August 2023. Concurrently, we expanded our experimental setup to assess harmaline-induced tremor in mice performing various motor tasks, such as high-speed locomotion, balancing, and 3D space exploration. Furthermore, we have integrated these intricate naturalistic behavior tasks with simultaneous recording of cerebellar nuclear activity, completing the initial full batch of experiments in April 2023. Our progress demonstrates our unwavering commitment to overcoming challenges in studying tremor-related disorders.

在过去的一年中，我们的专业研究团队在阐明震颤相关疾病的运动学和神经运动机制方面取得了显着进展。我们已经成功启动了一个项目，以确定 GCamP6 在下橄榄 (IO) 中病毒表达的安全条件，这对于了解骆驼蓬碱引起的小鼠震颤起着至关重要的作用。这项工作的研究结果将于 2023 年 8 月在仙台举行的著名 JNS 会议上公布。同时，我们扩展了实验设置，以评估执行各种运动任务（例如高速运动、平衡和 3D 空间探索）的小鼠中骆驼蓬碱引起的震颤。此外，我们将这些复杂的自然行为任务与小脑核活动的同步记录相结合，于 2023 年 4 月完成了最初的整批实验。我们的进展表明了我们坚定不移地致力于克服研究震颤相关疾病的挑战。