Identification of putative plant defense activators of novel mode of action.

鉴定新作用模式的假定植物防御激活剂。

• 批准号: 23380027
• 负责人: KUCHITSU Kazuyuki
• 金额: $ 12.31万
• 依托单位: Tokyo University of Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23380027/
• 关键词: 膜交通系; 活性酸素種; カルシウムイオン; 植物免疫; 植物防除剤; 植物免疫活性化剤; 抵抗性誘導剤; ケミカルバイオロジー; 陰イオンチャネル; サリチル酸; ジャスモン酸; オートファジー; シグナル伝達系; サリチル酸経路; 植物防疫剤; ジャスモン酸経路; 植物免役; タバコ培養細胞BY-2; エリシター; カルシウムチャネル; ファイトアレキシン; 植物免役活性化剤

Plant defense activators are chemicals that induce disease resistance by enhancing plant immunity upon pathogen infection. Defense signaling pathways induced by cryptogein, a proteinaceous elicitor derived from a pathogenic oomycete, in tobacco BY-2 cells has been extensively studied as a simple model system. Based on the correlation between the temporal pattern of enzymatic production of reactive oxygen species (ROS) and downstream defense responses, we established a new high-throughput screening method to identify novel lead compounds for plant defense activators. From a total of 11,000 compounds screened, we selected 58 candidates for activators. We further examined the effects of selected compounds on the expression of defense-related genes in Arabidopsis plants, and discovered some chemicals that potentially enhance jasmonic acid-dependent signaling pathway as putative plant defense activators with a novel mode of action.

植物防御激活剂是通过增强植物对病原体感染的免疫力来诱导抗病性的化学物质。烟草 BY-2 细胞中隐菌素（一种源自致病性卵菌的蛋白质激发子）诱导的防御信号通路已作为简单的模型系统进行了广泛研究。基于活性氧（ROS）酶促产生的时间模式与下游防御反应之间的相关性，我们建立了一种新的高通量筛选方法来鉴定植物防御激活剂的新型先导化合物。从总共筛选的 11,000 种化合物中，我们选择了 58 种候选激活剂。我们进一步研究了所选化合物对拟南芥植物中防御相关基因表达的影响，并发现了一些可能增强茉莉酸依赖性信号通路的化学物质，作为具有新颖作用模式的假定植物防御激活剂。

项目成果

Discovering Compounds That Activate Plant Immunity Using Machine Learning

使用机器学习发现激活植物免疫力的化合物

• 发表时间: 2014
• 作者: Yoshida T; Kitahata N; Ohwada H; Kuchitsu K
• 通讯作者: Kuchitsu K

Hedrich R, On the cellular site of two-pore channel TPC1 action 1 in the Poaceae

Hedrich R，禾本科双孔通道 TPC1 作用 1 的细胞位点

• DOI: 10.1111/nph.12402
• 发表时间: 2013
• 影响因子: 9.4
• 作者: Dadacz

Invivoimaging and quantitative monitoring of autophagic flux in tobacco BY-2 cells

烟草 BY-2 细胞自噬流的体内成像和定量监测

• DOI: 10.4161/psb.22510
• 发表时间: 2013
• 作者: Hanamata S; Kurusu T; Okada M; Suda A; Kawamura K; T sukada E; Kuchitsu K
• 通讯作者: Kuchitsu K

Plasma membrane protein OsMCA1 is involved in regulation of hypo-osmotic shock-induced Ca^ influx and modulates generation of reactive oxygen species in cultured rice cells

质膜蛋白 OsMCA1 参与调节低渗休克诱导的 Ca^2 内流并调节培养水稻细胞中活性氧的产生

• DOI: 10.1186/1471-2229-12-11
• 发表时间: 2012
• 影响因子: 5.3
• 作者: 来須孝光;西川大輔;山崎友香里;後藤真理子;中野正貴;濱田晴康;山中拓哉;飯田和子;中川祐子;佐治光;篠崎一雄;飯田秀利;朽津和幸
• 通讯作者: 朽津和幸

A novel calcium binding site in the slow vacuolar cation channel TPC1 senses luminal calcium level

慢液泡阳离子通道 TPC1 中的新型钙结合位点可感知管腔钙水平

• DOI: 10.1105/tpc.111.086751
• 发表时间: 2011
• 影响因子: 11.6
• 作者: Dadacz