Development of new gene transfer system for small fish

小型鱼类新基因转移系统的开发

• 批准号: 22659148
• 负责人: SAKAIDA Isao
• 金额: $ 2.04万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22659148/
• 关键词: 肝臓; 遺伝子導入; 線維化; 遺伝子導入法; ゼブラフィッシュ; メダカ; HTVi法; 肝臓病

The possibility of gene therapy for zebrafish and medaka with hydrodynamic methods was examined. A condition setup for gene transfer was performed with GFP plasmid. We found few GFP positive cells in the liver. Gene transfer efficiency was small compared with the infusion of the mouse models.We established fish live fibrosis model for evaluation of liver fibrosis improvement. We found that high fat diet and carbon tetrachloride is not suitable for liver fibrosis induction and DEN treatment for two months is suitable for liver fibrosis.

研究了利用流体动力学方法对斑马鱼和青鳉进行基因治疗的可能性。使用 GFP 质粒进行基因转移的条件设置。我们在肝脏中发现了很少的 GFP 阳性细胞。与小鼠模型的输注相比，基因转移效率较小。我们建立了鱼活纤维化模型来评价肝纤维化的改善情况。我们发现高脂肪饮食和四氯化碳不适合诱发肝纤维化，而DEN治疗两个月适合肝纤维化。

项目成果

SMP30/ Regucalcin is a useful marker for liver tumor ISHSR

SMP30/Reguccalcin 是肝脏肿瘤的有用标记物 ISHSR

• 发表时间: 2011
• 作者: Koichi Fujisawa; Shuji Terai; Taro Takami; Naoki Yamamoto; Isao Sakaida
• 通讯作者: Isao Sakaida

Senescence marker protein 30 (SMP30)/regucalcin (RGN) expression decreases with aging, acute liver injuries and tumors in zebrafish

斑马鱼衰老标记蛋白 30 (SMP30)/regcalcin (RGN) 表达随着衰老、急性肝损伤和肿瘤而降低

• DOI: 10.1016/j.bbrc.2011.09.067
• 发表时间: 2011
• 作者: Fujisawa K

Telmisartan improved nonalcoholic steatohepatitis in medaka (Oryzias latipes) by reducing macrophage infiltration and fat accumulation.

替米沙坦通过减少巨噬细胞浸润和脂肪积累来改善青鳉 (Oryzias latipes) 的非酒精性脂肪性肝炎。

• 发表时间: 2011
• 作者: Kuwashiro S

Senescence marker protein 30(SMP30)/ regucalcin(RGN) expression decreases with aging, acute liver injuries and tumors in zebrafish

斑马鱼衰老标志蛋白30（SMP30）/调节钙素（RGN）表达随着衰老、急性肝损伤和肿瘤而降低

• 发表时间: 2011
• 作者: Fujisawa K; Terai S; Hirose Y; Takami T; Yamamoto N; Sakaida I.
• 通讯作者: Sakaida I.

Fish model leads to new findings in liver disease.

鱼模型带来了肝脏疾病的新发现。

• 发表时间: 2010
• 影响因子: 4.2
• 作者: Terai S