In vivo imaging of sensory synaptic inputs in neocortex

新皮质感觉突触输入的体内成像

• 批准号: 22650083
• 负责人: KITAMURA Kazuo
• 金额: $ 2.13万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22650083/
• 关键词: ニューロン; シナプス; 2光子イメージング; シナプス統合; 樹状突起; 体性感覚

We performed simultaneous whole-cell patch-clamp recordings and two-photon calcium imaging in layer 2/3 of mouse barrel cortex in vivo. Calcium signals in individual spines of layer 2/3 pyramidal cells induced by spontaneous and sensory-evoked activities could be clearly observed. We found that approximately 80% of synaptic inputs were localized only in 25% of total spines. Furthermore, we found that nearby spines were tended to be activated simultaneously. Thus, our results suggest that functionally coupled neurons innervate to nearby spines in dendrites of postsynaptic pyramidal neurons.

我们在体内小鼠桶状皮层 2/3 层中同时进行全细胞膜片钳记录和双光子钙成像。可以清楚地观察到自发和感觉诱发活动诱导的 2/3 层锥体细胞单个棘中的钙信号。我们发现大约 80% 的突触输入仅位于总棘的 25% 中。此外，我们发现附近的刺往往会同时被激活。因此，我们的结果表明，功能耦合神经元支配突触后锥体神经元树突中附近的棘。

项目成果

Heterogeneous organization of sensory synaptic inputs in the mouse barrel cortex

小鼠桶状皮层感觉突触输入的异质组织

• 发表时间: 2011
• 作者: Kazuo Kitamura

Two-photon imaging of Purkinje cell dendritic activity in vivo

体内浦肯野细胞树突活性的双光子成像

• 发表时间: 2011
• 作者: Kazuo Kitamura

Simultaneous two-photon calcium imaging and patch-clamp recordings in vivo

体内同时双光子钙成像和膜片钳记录

• 发表时间: 2010
• 作者: K.Kitamura; M.Hausser; M.Kano
• 通讯作者: M.Kano

Locally synchronized synaptic inputs

局部同步突触输入

• DOI: 10.1126/science.1210362
• 发表时间: 2012
• 影响因子: 56.9
• 作者: Takahashi N; Kitamura K; Matsuo N; Mayford M; Kano M; Matsuki N; Ikegaya Y
• 通讯作者: Ikegaya Y

Enhanced synchrony of climbing fiber-induced Ca2+signaling in cerebellar Purkinje cells caused by elevated synchronous activity of inferior olivary neurons in GluD2(GluRδ2) knockout mouse

GluD2(GluRδ2) 敲除小鼠下橄榄神经元同步活性升高导致小脑浦肯野细胞攀爬纤维诱导的 Ca2+ 信号传导增强同步

• 发表时间: 2011
• 作者: Miki Hashizume; Kazuo; Kitamura; Kenji Sakimura;Masanobu Kano
• 通讯作者: Masanobu Kano