A genetical screening of a protein trap library for active chromatin-binding factors on polytene chromosome in Drosophila

果蝇多线染色体上活性染色质结合因子的蛋白质陷阱文库的遗传筛选

• 批准号: 22770166
• 负责人: SAWATSUBASHI Shun
• 金额: $ 2.75万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22770166/
• 关键词: 転写; クロマチン; 核内受容体; エクダイソン; エクダイソンレセプター; 転写制御

It is evident that gene regulation is coupled with chromatin reorganaization, but factors and molecular basis involving in this process remain to be identified. In the present study, factors responsible for ecdysone-induced puff formation were genetically screened in Drosophila fly by establishing an EGFP protein-trap approach. Among the candidates, Drosophila CG11138/Ringfect was found as a co-activator for ecdysone receptor (EcR). Through biochemical characterization, Ringfect could be shown to serve as a histone chaperon. We describe the identification of novel puff-localized factors by screening the protein trap transgenic fly library. Drosophila CG11138/Ringfect and enolase were genetically identified as a puff-localized factor and a co-activator, and interacted with EcR in S2 cell. Thus, the present study suggests that Ringfect and enolase are categorized as a novel class of nuclear receptor co-activators.

很明显，基因调控与染色质重组相结合，但参与这一过程的因素和分子基础仍有待确定。在本研究中，通过建立 EGFP 蛋白捕获方法，在果蝇中对导致蜕皮激素诱导的粉扑形成的因素进行了基因筛选。在候选者中，果蝇 CG11138/Ringfect 被发现是蜕皮激素受体 (EcR) 的共激活剂。通过生化表征，Ringfect 可作为组蛋白伴侣。我们描述了通过筛选蛋白质陷阱转基因果蝇文库来鉴定新型粉扑局部因子。果蝇 CG11138/Ringfect 和烯醇化酶被基因鉴定为喷发定位因子和共激活剂，并与 S2 细胞中的 EcR 相互作用。因此，本研究表明 Ringfect 和烯醇酶被归类为一类新型核受体共激活剂。

项目成果

CDP/cut is an osteoblastic coactivator of the vitamin D receptor (VDR).

CDP/cut 是维生素 D 受体 (VDR) 的成骨细胞共激活剂。

• 发表时间: 2010
• 作者: 沢津橋俊

Epigenetic silencing of core histone genes by HERS in drosophila

果蝇中 HERS 对核心组蛋白基因的表观遗传沉默

• 发表时间: 2012
• 影响因子: 16
• 作者: Ito S; Fujiyama
• 通讯作者: Fujiyama

Epigenetic silencing of core histone genes by HERS in Drosophila.

果蝇中 HERS 对核心组蛋白基因的表观遗传沉默。

• DOI: 10.1016/j.molcel.2011.12.029
• 发表时间: 2012
• 影响因子: 16
• 作者: Ito S.; et al.
• 通讯作者: et al.

DEKはヒストンシャペロンとして機能する新規転写活性化共役因子である

DEK 是一种新型转录激活辅助因子，可充当组蛋白伴侣

• 发表时间: 2011
• 作者: 沢津橋俊

炎症とエネルギー代謝の切り換えを担う未知核内エピゲノム制御メカニズムの探索

寻找负责炎症和能量代谢之间切换的未知核表观基因组调控机制

• 发表时间: 2011
• 作者: 沢津橋俊