Searching for main clock in plants

寻找植物的主时钟

• 批准号: 22770036
• 负责人: ENDO Motomu
• 金额: $ 2.75万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22770036/
• 关键词: 生物時計; 組織特異性; シロイヌナズナ

To elucidate tissue-specific functions of biological clocks in plants, we transformed and expressed TOC1-GFP or CCA1-GFP under several different tissue/organ-specific promoters, and suppressed biological clock functions tissue-specific manners. Some of transgenic lines showed late flowering phenotype and long hypocotyl length. Furthermore, by using split luciferase, we established reporter constructs that can detect clock promoters activity with tissue specific manners.

为了阐明植物生物钟的组织特异性功能，我们在几种不同的组织/器官特异性启动子下转化并表达TOC1-GFP或CCA1-GFP，并以组织特异性方式抑制生物钟功能。一些转基因品系表现出晚花表型和长下胚轴长度。此外，通过使用裂解荧光素酶，我们建立了报告基因构建体，可以以组织特异性方式检测时钟启动子活性。