Simultaneous sensitive detection of chelated medicines and poisonsby electrospray ionization tandem mass spectrometry without column separation

电喷雾电离串联质谱无需柱分离同时灵敏检测螯合药物和毒物

• 批准号: 22590631
• 负责人: MINAKATA Kayoko
• 金额: $ 2.91万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590631/
• 关键词: 薬毒物; 錯体; タンデム質量分析; イオンスプレー; 質量分析; 過塩素酸; ヨード; 金; 銅; アジ化ナトリウム; Au; 錯体化合物; シアン; 胃内容; 尿; 血液; 練炭中毒

To facilitate the detection of highly hydrophilic medicines and poisons, we developed a method that produces chelate complexes of analytes and then extracts them with polar organic solvents before electrospray ionization mass spectrometric (ESI-MS) detection. During the study we noticed the possibility to detect several analytes simultaneously by the method. In the simultaneous detection, usually, column separation was performed to separate analytes as well as to eliminate impurities. In tandem mass spectrometry (MS -MS), MS detection was performed twice in a blink for precursor ion and product ion, and hence impurities were eliminated twice. Most analytes having different molecular structures showed different product ions at the 2ndMS even though they showed the same m/z at the 1stMS. Therefore, our simultaneous detection method by ESI-MS-MS did not requirecolumn separation that took about 30 min. Using this method, three groups of analytes, that is, 1. cyanide-azide ion group, 2. coinage-metal group, 3. ion group that related to hypothyroidism, could be detected simultaneously during these three years. Multi analytes detection using a single sample without column separation contributes greatly to the saving of time and resources in forensic and clinical toxicology.

为了促进高亲水性药物和毒物的检测，我们开发了一种方法，产生分析物的螯合物，然后用极性有机溶剂萃取它们，然后进行电喷雾电离质谱（ESI-MS）检测。在研究过程中，我们注意到通过该方法可以同时检测多种分析物。在同时检测中，通常进行柱分离以分离分析物并消除杂质。在串联质谱(MS-MS)中，母离子和产物离子在眨眼间进行两次MS检测，因此两次消除杂质。大多数具有不同分子结构的分析物在第 2 次 MS 中显示出不同的产物离子，尽管它们在第 1 次 MS 中显示出相同的 m/z。因此，我们的 ESI-MS-MS 同步检测方法不需要大约 30 分钟的柱分离。利用该方法，三年内可同时检测出三组分析物，即1.氰化物-叠氮化物离子组、2.货币-金属组、3.与甲状腺功能减退症相关的离子组。使用单个样品进行多分析物检测而无需进行柱分离，极大地节省了法医和临床毒理学的时间和资源。

项目成果

ESI-MS-MSによる胃内容・尿中アジ化物イオンの迅速・高感度定量法

使用 ESI-MS-MS 定量胃内容物和尿叠氮化物离子的快速、高灵敏度方法

• 发表时间: 2011
• 作者: 南方かよ子;野澤秀樹;他 6 名
• 通讯作者: 他 6 名

Analysis of ibotenic acid and muscimol in Amanitamushrooms by hydrophilic interaction liquid chromatography-tandem mass spectrometry.

亲水作用液相色谱-串联质谱法分析鹅膏菌中的鹅膏酸和蝇蕈醇。

• DOI: 10.1007/s11419-012-0144-7
• 发表时间: 2012
• 影响因子: 2.2
• 作者: Kunio Gonmori; Koutaro Hasegawa; Hiroki Fujita; Yoshito Kamijo; Hideki Nozawa; Itaru Yamagishi; Kayoko Minakata; Kanako Watanabe; Osamu Suzuki
• 通讯作者: Osamu Suzuki

Simultaneous analysis of cardiac glycosides in blood and urine by thermoresponsive LC-MS-MS

热响应 LC-MS-MS 同时分析血液和尿液中的强心苷

• DOI: 10.1007/s00216-010-4405-1
• 发表时间: 2011
• 作者: Sanae Kanno; Kanako Watanabe; Itaru Yamagishi; Seishiro Hirano; Kayoko Minakata; Kunio Gonmori; Osamu Suzuki
• 通讯作者: Osamu Suzuki

A sensitive and fully automated method for GC-MS analysis of volatile organic compounds in whole blood using large volumes of headspace vapor.

一种灵敏且全自动的方法，使用大量顶空蒸汽对全血中的挥发性有机化合物进行 GC-MS 分析。

• 发表时间: 2012
• 作者: Koutaro Hasegawa; Amin Wurita; Kunio Gonmori; Hideki Nozawa; Itaru Yamagishi; Kayoko Minakata; Osamu Suzuki; Kanako Watanabe
• 通讯作者: Kanako Watanabe

MALDI-TOF mass spectrometric determination of 11 phenothiazines with heavy side chains in urine

MALDI-TOF质谱法测定尿液中11种重侧链吩噻嗪类药物

• DOI: 10.1007/s11419-012-0157-2
• 发表时间: 2013
• 影响因子: 2.2
• 作者: Kayoko Minakata; Hideki Nozawa
• 通讯作者: Hideki Nozawa