Osteoinductivity of gelatin/β-tricalcium phosphate sponges loaded with different concentrations of mesenchymal stem cells and bone morphogenetic protein-2 in an equine bone defect.

负载不同浓度间充质干细胞和骨形态发生蛋白2的明胶/β-磷酸三钙海绵在马骨缺损中的骨诱导性。

• 批准号: 22580360
• 负责人: SASAKI Naoki
• 金额: $ 2.91万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580360/
• 关键词: 再生医療; 骨; 関節軟骨; ゼラチンハイドロゲル; 骨形成蛋白; 多血小板血漿; 生体組織工学; 足場; ドラックデリバリーシステム; 幹細胞; ウマ; 骨折; 軟骨; 骨スクリュー; スキヤフォールド

β-TCP sponge was an excellent scaffold material, which builds a DDS for BMP-2. Based on these results, it was shown that the combined use of growth factors and scaffolds is effective in bone regeneration. it was suggested that the combined use of MSC, BMP-2 and □-TCP sponge efficiently promoted bone regeneration. Based on the results of the five studies, it was demonstrated that the application of tissue engineering combining growth factors, cells, and scaffolds promoted bone and articular cartilage regeneration. Therefore, future clinical applications to bone and articular cartilage diseases of horses are expected.

β-TCP海绵是一种优秀的支架材料，为BMP-2构建了DDS。基于这些结果，表明生长因子和支架的联合使用对于骨再生是有效的。 MSC、BMP-2和□-TCP海绵可有效促进骨再生。五项研究的结果表明，结合生长因子、细胞和支架的组织工程的应用可促进骨和关节软骨的再生。因此，未来有望应用于马骨和关节软骨疾病的临床应用。

项目成果

Comparison of allogeneic platelet lysate and fetal bovine serum for in vitro expansion of equine bone marrow-derived mesenchymal stem cells.

同种异体血小板裂解物和胎牛血清体外扩增马骨髓间充质干细胞的比较。

• 发表时间: 2013
• 影响因子: 2.4
• 作者: Jong

馬の距骨外側滑車の関節軟骨欠損に対する生体組織工学を用いた2層法の影響

利用组织工程的两层方法对马外侧滑车关节软骨缺损的影响

• 发表时间: 2012
• 作者: 田邊貴史;都築直;徐鐘筆;羽田真悟;古岡秀文;山田一孝;田畑泰彦;佐々木直樹
• 通讯作者: 佐々木直樹

The effect of gelatin hydrogel sheet containing bFGF on proximal sesamoid bone transverse fracture in the horse

含bFGF的明胶水凝胶片对马近端籽骨横向骨折的影响

• 发表时间: 2013
• 作者: Naoki Sasaki; Satoru Nishii; Kazutaka Yamada; Hidehumi Huruoka; Yasuhiko Tabata
• 通讯作者: Yasuhiko Tabata

The effect of bone morphogenetic protein-2, platelet-rich-plasma incorporated gelatin hydrogel microsphere, mesenchymal stem cell and gelatin ß-tricalcium phosphate sponge on equine articular cartilage defect.

骨形态发生蛋白-2、富含血小板血浆的明胶水凝胶微球、间充质干细胞和明胶β-磷酸三钙海绵对马关节软骨缺损的影响。

• 发表时间: 2013
• 作者: Nao Tsuzuki; Jong
• 通讯作者: Jong

Proliferation of equine bone marrow-derived mesenchymal stem cells in gelatin/β-tricalcium phosphate sponges

马骨髓间充质干细胞在明胶/β-磷酸三钙海绵中的增殖

• 发表时间: 2012
• 作者: Jong