Cellular and Immune Responses of Dairy Cows Induced by Intramammary Infusion of Lactic Acid Bacteria and Therapeutic Trials to Quarters of Cows with Mastitis.

乳房内输注乳酸菌诱导的奶牛的细胞和免疫反应以及对四分之一患有乳腺炎的奶牛的治疗试验。

• 批准号: 22580354
• 负责人: NAGAHATA Hajime
• 金额: $ 2.91万
• 依托单位: Rakuno Gakuen University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580354/
• 关键词: 乳房炎; 有用微生物; 白血球機能修飾; サイトカイン; 乳腺感染制御; 乳酸菌; 乳体細胞の誘導; 乳房内注入; サイトカイン発現; 感染制御効果; Bifidobacterium breve; 感染防御応答; 白血球機能; 表面マーカ

The aim of this study was to characterize the cellular and immune responses of mammary gland by intramammary infusion (IMI) of Lactic acid bacteria (LAB) in cows, and to evaluate the therapeutic effects of IMI of LAB to lactating cows withmastitis. Marked increase of SCC were found in infused quarter at 24-48 hr post infusion. Significantly increased concentrations of immunoglobulins, anti-bacterial proteins and mRNA expression of cytokines on milk leukocytes were found in cows. In therapeutic trials, IMI of LAB to quarters leads to a marked cellular and immune responses in mammary gland. The IMI of LAB may have a possible rolefor control of mastitis.(key words:lactic acid bacteria, mammary gland defense, immune-therapeutic trial, bovine mastitis control)

本研究的目的是通过乳腺内输注乳酸菌（LAB）来表征奶牛乳腺的细胞和免疫反应，并评估 LAB IMI 对泌乳奶牛乳腺炎的治疗效果。在输注后 24-48 小时，输注季度发现 SCC 显着增加。奶牛的乳白细胞中免疫球蛋白、抗菌蛋白和细胞因子 mRNA 表达的浓度显着增加。在治疗试验中，将 LAB 注射到四分之一的 IMI 会导致乳腺产生显着的细胞和免疫反应。 LAB的IMI可能对控制乳腺炎有一定的作用。（关键词：乳酸菌，乳腺防御，免疫治疗试验，牛乳腺炎控制）

项目成果

Changes in acute-phase proteins and cytokines in serum and milk whey from dairy cows with naturally occurring peracute mastitis caused by Klebsiella pneumonia and the relationship to clinical outcome

肺炎克雷伯菌引起的自然发生的超急性乳腺炎奶牛血清和乳清中急性期蛋白和细胞因子的变化及其与临床结局的关系

• 发表时间: 2011
• 作者: Hisaeda; K.; Arima; H.; Sonobe; T.; Nasu; M.; Hagiwara; K.; Kirisawa; R.; Takahashi; T.; Kikuchi; N. & Nagahata; H
• 通讯作者: H

Analysis of cellular and immune responses of dairy cows induced by intramammary infusion of lactic acid bacteria and therapeutic trials to quarters of cows with mastitis

乳房内输注乳酸菌引起的奶牛细胞和免疫反应分析以及对四分之一乳腺炎奶牛的治疗试验

• 发表时间: 2012
• 作者: Nagahata; H.; Moriyama; A.; Koshi; A.; Mukai; T.; Natsume; Y.; Higuchi; T.; Koike; N. & Ando; T
• 通讯作者: T

Characterization of novel Staphylococcus aureus bacteriophages with wide host ranges and their potential as therapeutic agents for bovine mastitis

具有广泛宿主范围的新型金黄色葡萄球菌噬菌体的表征及其作为牛乳腺炎治疗剂的潜力

• 发表时间: 2012
• 作者: Iwano; H.; Higuchi; H.; Inoue; H.; Kobayashi; H.; Nagahata; H.; Yokota; H. & Tanji; Y
• 通讯作者: Y

Altered leukocyte responsiveness in dairy cows with naturally occurring chronic Staphylococcus aureus mastitis

患有自然发生的慢性金黄色葡萄球菌乳腺炎的奶牛白细胞反应性改变

• 发表时间: 2011
• 影响因子: 1.2
• 作者: Nagahata; H.; Kawai; H.; Higuchi; H.; Kawai; K.; Chang; C.J.
• 通讯作者: C.J.

Analysis of cellular and immune responses of dairy cows induced by intramammary infusion of lactic acid bacteria and therapeutic trials to quarters of cows with mastitis.

乳房内输注乳酸菌引起的奶牛细胞和免疫反应分析以及对四分之一乳腺炎奶牛的治疗试验。

• 作者: Nagahata; H. et al (8)
• 通讯作者: H. et al (8)