Structural changes accompanying mitigation of the allergic action ofovomucoid after passing an electric current

通过电流后伴随卵类粘蛋白过敏作用减轻的结构变化

• 批准号: 22580138
• 负责人: MATSUMOTO Tomoaki
• 金额: $ 2.58万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580138/
• 关键词: 卵アレルギー; 卵アレルゲン; 低アレルゲン化; オボムコイド; 直流電流; プロテオミクス; 蛋白高次構造; 食物アレルギー; 通電処理; 低アレルゲン食品; 質量分析; 鶏卵アレルギー; 卵白オボムコイド; 卵白アレルゲン; 蛋白質量分析

Ovomucoid is the most prominent allergen causing hen’s eggallergy, containing S-S bonds that may be responsible for its allergic action. Theallergic action of this protein has been reduced after electrolysis on both the anodeand the cathode sides when evaluated by mean of skin prick. The results for modificationsof ovomucoid after electrolysis showed two distinct peptide fragments （57E-63K、123H-128R）had lost with matrix-associated laser desorption/ionization time-of-flight massspectrometry. Although the total free SH groups in ovomucoid were increased on thecathode side, the regions for breaking S-S bonds have not been determined in this study

卵类粘蛋白是引起母鸡鸡蛋过敏的最重要的过敏原，其含有可能导致其过敏作用的S-S键，当通过皮肤刺痛进行评估时，该蛋白质的过敏作用在阳极和阴极两侧均被降低。电解后卵类粘蛋白的修饰显示两个不同的肽片段（57E-63K、123H-128R）因基质相关激光而丢失解吸/电离飞行时间质谱虽然卵类粘蛋白中游离SH基团的总量在阴极侧有所增加，但本研究尚未确定断裂S-S键的区域。

项目成果

Mitigation of the allergic activity of ovomucoid by electrolysis

通过电解减轻卵类粘蛋白的过敏活性

• 发表时间: 2012
• 作者: 松本知明

Mitigation of the allergicaction of ovomucoid with electricalenergy

用电能减轻卵类粘蛋白的过敏反应

• 发表时间: 2012
• 作者: Matsumoto T