CLEC2-PLAG complex-induced oncogenic transformation in the intra-lymphatic microenvironment

CLEC2-PLAG 复合物诱导淋巴内微环境致癌转化

• 批准号: 22390345
• 负责人: SAWA Yoshihiko
• 金额: $ 12.73万
• 依托单位: Fukuoka Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390345/
• 关键词: 口腔解剖学(含組織学発生学); 微小循環; 口腔扁平上皮癌; ポドプラニン; 抗体; 転移; 細胞接着; リンパ管内皮細胞; 血小板; CLEC2; PMab-1; NZ-1; 唾液腺; 脈絡叢; PLAG; 癌幹細胞; 遺伝子操作マウス

We developed novel antibodies specific for human and mouse podoplanin (PMab-1,NZ-1.2) sold in MBL, Imgenex, Sigma, and Millipore. Immunohistochemical studies on the oral squamous cell carcinoma (SCC) revealed that platelets exist between SCC and lymphatic endothelial cells at the luminal side of lymphatic vessels where SCC attached. The platelets may contribute to the lymphogenous metastasis of SCC. The adhesion activity to human lymphatic endothelial cells was higher in podoplanin-positive SCC than in podoplanin-negative SCC, and the adhesion of SCC was blocked by NZ-1.2. Anti-podoplanin may be useful for the inhibition of the lymphogenous metastasis of SCC.

我们开发了针对人和小鼠足足蛋白 (PMab-1,NZ-1.2) 的新型抗体，在 MBL、Imgenex、Sigma 和 Millipore 出售。对口腔鳞状细胞癌（SCC）的免疫组织化学研究表明，血小板存在于SCC和SCC附着的淋巴管管腔侧的淋巴管内皮细胞之间。血小板可能有助于鳞状细胞癌的淋巴转移。平足蛋白阳性SCC对人淋巴管内皮细胞的粘附活性高于平足蛋白阴性SCC，并且NZ-1.2阻断了SCC的粘附。抗平足蛋白可能有助于抑制鳞状细胞癌的淋巴转移。

项目成果

Expression of Toll-Like Receptor 4 in Glomerular Endothelial Cells under Diabetic Conditions.

糖尿病条件下肾小球内皮细胞中 Toll 样受体 4 的表达。

• 发表时间: 2013
• 作者: Takata S; Sawa Y
• 通讯作者: Sawa Y

Immunohistochemical examination of novel rat monoclonal antibodies against mouse and human podoplanin.

针对小鼠和人足足蛋白的新型大鼠单克隆抗体的免疫组织化学检查。

• 发表时间: 2012
• 作者: Kaji C; Tsujimoto Y; Kaneko; MK; Kato Y;Sawa Y.
• 通讯作者: Sawa Y.

The expression of podoplanin and classic cadherins in the mouse brain

小鼠大脑中足足蛋白和经典钙粘蛋白的表达

• 发表时间: 2012
• 影响因子: 2.4
• 作者: Kaji C; Tomooka M; Kato Y; Kojima H; Sawa Y
• 通讯作者: Sawa Y

Fibrillin-1 and fibrillin-2 are essential for formation of thick oxytalan fibers in human nonpigmented ciliary epithelial cells in vitro

Fibrillin-1 和 fibrillin-2 对于体外人非色素性睫状上皮细胞中厚氧化纤维的形成至关重要

• 发表时间: 2012
• 影响因子: 2.9
• 作者: Yamanouchi K; Tsuruga E; Oka K; Sawa Y; Ishikawa H
• 通讯作者: Ishikawa H

Growth Differentiation Factor 5 induces matrix metalloproteinase 2 expression in periodontal ligament cells and modulates MMP-2 and MMP-13 activity in osteoblasts.

生长分化因子 5 诱导牙周膜细胞中基质金属蛋白酶 2 的表达，并调节成骨细胞中 MMP-2 和 MMP-13 的活性。

• 发表时间: 2011
• 作者: Hatakeyama Y;Oka K;Tsuruga E;Sawa Y.
• 通讯作者: Sawa Y.