Development of delivery systems of functional food factors by vesiculation and modulation of transepithelial transport

通过囊泡化和跨上皮运输调节开发功能性食品因子的输送系统

• 批准号: 21780138
• 负责人: WATANABE Jun
• 金额: $ 3万
• 依托单位: National Agricultural Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21780138/
• 关键词: ナノスケール化; 細胞内輸送; フラボノイド; Caco-2細胞; ヘスペレチン; ベシクル

In this research, we aimed to enhance the absorption of functional food factors, especially flavonoids, by optimization of vasiculation condition of flavonoids and by modulation of transepithelial transport in the intestine. When flavonoids were incorporated to vesicles, flavonoids were stably suspended beyond their saturated concentrations, and quercetin was protected from degradation in aqueous media. Permeated hesperetin across the Caco-2 cell monolayer was significantly increased when hesperetin coexisted with lipid vesicles. Several genes related to intracellular transport were down- or up-regulated by the treatment with dipeptide Trp-Gly in Caco-2 cells, suggesting that these genes possibly become the target for modulation of transepithelial transport by food components.

在这项研究中，我们的目的是通过优化类黄酮的血管条件和调节肠道内的跨上皮转运来增强功能性食品因子，特别是类黄酮的吸收。当类黄酮掺入囊泡时，类黄酮稳定地悬浮在超出其饱和浓度的位置，并且槲皮素在水介质中免受降解。当橙皮素与脂质囊泡共存时，透过 Caco-2 细胞单层的橙皮素显着增加。在 Caco-2 细胞中，用二肽 Trp-Gly 处理后，一些与细胞内转运相关的基因被下调或上调，这表明这些基因可能成为食物成分调节跨上皮转运的靶标。

项目成果

フラボノイドに対する脂質ベシクルの安定化効果

脂囊泡对黄酮类化合物的稳定作用

• 发表时间: 2011
• 作者: 大泉祐樹; ら
• 通讯作者: ら

フラボノイドに対する脂質ベシクルの安定化効果

脂囊泡对黄酮类化合物的稳定作用

• 作者: 大泉祐樹;池山明日香;喜久川真記;佐藤誠吾;渡辺純;市川創作
• 通讯作者: 市川創作

Preparation and characterization of lipid vesicles containing a hydrophobic flavonoid

含有疏水性黄酮类化合物的脂质囊泡的制备和表征

• 发表时间: 2009
• 作者: Asuka Ikeyama; Maki Kikugawa; Seigo Sato; Jun Watanabe; Sosaku Ichikawa
• 通讯作者: Sosaku Ichikawa

Preparation and characterization of lipid vesicles containing a hydrophobic flavonoid

含有疏水性黄酮类化合物的脂质囊泡的制备和表征

• 作者: Asuka Ikeyama; Maki Kikugawa; Seigo Sato; Jun Watanabe; Sosaku Ichikawa
• 通讯作者: Sosaku Ichikawa

脂質ベシクル共存下におけるフラボノイドのCaco-2細胞単層膜透過特性

脂质囊泡存在下黄酮类化合物的 Caco-2 细胞单层膜渗透特性

• 发表时间: 2010
• 作者: 池山明日香; ら
• 通讯作者: ら