Ecological study on marine luminous bacteria possessing proteorhodopsin

具有蛋白视紫红质的海洋发光细菌的生态学研究

• 批准号: 21380123
• 负责人: KOGURE Kazuhiro
• 金额: $ 11.4万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21380123/
• 关键词: プロテオロドプシン; 海洋細菌; 光エネルギー; プロトンポンプ活性; Flavobacteria; 遺伝子解析; 遺伝子伝搬; 16S rDNA; Bacteroidetes; エルギー代謝; 海洋生態系; 系統解析; エネルギー代謝

Proteorhodopsin (PR) is a membrane protein that absorbs light energy and functions as proton pump. The proton gradient thus established enables the cells to synthesize ATP, thus serves as efficient energy synthesis component. This was first reported in 2000. Since then there have been estimated that nearly half of prokaryotes present in the upper water column in the ocean may possess PR. However, so far only about 10 isolates had been reported to possess PR, that makes any further physiological investigations difficult. Our group succeeded to isolate more than 60 strains and directly measure the activity of proton pump under light. In addition, with further assumptions we succeeded to estimate the contribution of PR to light energy absorption to the ocean for the first time. The research on PR is now expanding after our investigation using isolates.

蛋白视紫红质 (PR) 是一种膜蛋白，可吸收光能并起到质子泵的作用。由此建立的质子梯度使细胞能够合成ATP，从而作为有效的能量合成成分。这是在 2000 年首次报道的。从那时起，人们估计海洋上层水体中近一半的原核生物可能拥有 PR。然而，到目前为止，据报道只有大约 10 个分离株具有 PR，这使得进一步的生理学研究变得困难。我们课题组成功分离出60余株菌株，并直接测量光下质子泵的活性。此外，通过进一步的假设，我们首次成功估计了 PR 对海洋光能吸收的贡献。在我们使用分离株进行调查后，PR 研究现在正在扩大。

项目成果

Diversity and functional analysis of proteorhodopsin in marine Flavobacteria

海洋黄杆菌中蛋白视紫红质多样性及功能分析

• DOI: 10.1111/j.1462-2920.2012.02702.x
• 发表时间: 2012
• 作者: Yoshizawa; S.; Kawanabe; A.; Ito; H.; K;ori; H.; Kogure; K.
• 通讯作者: K.

Vibrio jasicida sp. nov., a member of the Harveyi clade, isolated from marine animals (packhorse lobster, abalone and Atlantic salmon).

贾西弧菌 sp.

• DOI: 10.1099/ijs.0.025916-0
• 发表时间: 2012-08-01
• 期刊: International journal of systematic and evolutionary microbiology
• 影响因子: 2.8
• 作者: Susumu Yoshizawa;Yasuhiro Tsuruya;Y. Fukui;T. Sawabe;A. Yokota;K. Kogure;M. Higgins;J. Carson;F. Thompson
• 通讯作者: F. Thompson

海洋性フラボバクテリアのプロテオロドプシンによる光エネルギー利用

海洋黄细菌蛋白视紫红质对光能的利用

• 发表时间: 2011
• 作者: 吉澤晋

Proteorhodopsin is functioning as the light-driven proton pump among marine Flavobacteria

变形视紫红质在海洋黄细菌中充当光驱动质子泵

• 发表时间: 2010
• 作者: Yoshizawa; Susumu; Akira Kawanabe; Hideki K;ori;Kazuhiro Kogure
• 通讯作者: Kazuhiro Kogure

"Photoheterotrophy" -an overlooked mechanism of marine bacteria

“光异养”——海洋细菌被忽视的机制

• 发表时间: 2011
• 作者: Kogure Kazuhiro