Structural biology for elucidation of lysine biosynthesis and its regulatory mechanisms

阐明赖氨酸生物合成及其调控机制的结构生物学

• 批准号: 21380057
• 负责人: NISHIYAMA Makoto
• 金额: $ 12.23万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21380057/
• 关键词: 微生物酵素; 構造生物学; リジン生合成; ロイシン生合成; 超好熱性古細菌; アルギニン生合成; 機能分化; キャリアタンパク質; X線結晶構造解析; フィードバック阻害; ホモクエン酸合成酵素; アスパラギン酸キナーゼ

There are two different pathways in lysine biosynthesis. In this study, we have succeeded in elucidation of regulatory mechanisms of enzymes, each of which has a key role in each biosynthetic pathway. In the second half of lysine biosynthetic pathway throughα-aminoadipate, we discovered that a protein named LysW is not only used for modification of the amino group of the substrate, but also served as a carrier protein for biosynthetic enzymes.

赖氨酸生物合成有两种不同的途径，在这项研究中，我们成功地阐明了酶的调节机制，每种酶在通过α-氨基己二酸的赖氨酸生物合成途径的后半部分中都起着关键作用。发现一种名为LysW的蛋白质不仅用于修饰底物的氨基，而且还可以作为生物合成酶的载体蛋白。

项目成果

Structural basis for leucine-induced allosteric activation of glutamate dehydrogenase

亮氨酸诱导的谷氨酸脱氢酶变构激活的结构基础

• 发表时间: 2011
• 影响因子: 4.8
• 作者: T. Tomita; T. Kuzuyama;M. Nishiyama
• 通讯作者: M. Nishiyama

Enhancement of latent 3-isopropylmalate dehydrogenase activity of promiscuous homoisocitrate dehydrogenase by directed evolution

通过定向进化增强混杂高异柠檬酸脱氢酶的潜在 3-异丙基苹果酸脱氢酶活性

• 发表时间: 2010
• 影响因子: 4.1
• 作者: Y. Suzuki; K. Asada; J. Miyazaki; T. Tomita; T. Kuzuyama;M. Nishiyama
• 通讯作者: M. Nishiyama

Enhancement of substrate recognition and insight into feedback inhibition of homocitrate synthase from Thermus thermophilus

增强底物识别并深入了解嗜热栖热菌高柠檬酸合酶的反馈抑制

• 发表时间: 2010
• 作者: T.Okada; ら
• 通讯作者: ら

Mechanism for multiple-substrates recognition of α-aminoadipate aminotransferase from Thermus thermophilus

嗜热栖热菌α-氨基己二酸转氨酶的多底物识别机制

• 发表时间: 2009
• 作者: Tomita; T.; et al.
• 通讯作者: et al.

Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase from Thermus thermophilus

嗜热栖热菌 Thr 敏感天冬氨酸激酶调节亚基的晶体结构

• 发表时间: 2009
• 作者: Yoshida; A.; et al.
• 通讯作者: et al.