Analysis of GRWD1, a novel histone-binding protein involved in chromosomal replication initiation and segregation

GRWD1 的分析，一种参与染色体复制起始和分离的新型组蛋白结合蛋白

• 批准号: 21370084
• 负责人: FUJITA Masatoshi
• 金额: $ 8.99万
• 依托单位: Kyushu University (2010-2011)National Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East (2009)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21370084/
• 关键词: GRWD1; Cdt1; 複製開始複合体; 細胞周期; ヒストンシャペロ; MCM; クロマチン; 次世代シークエンシング; 複製開始制御; ヒストンシャペロン; ライセンシング制御; 複製開始点

Precise regulation of DNA replication reaction is essential for integrity of genome information. Cdt1, together with ORC and CDC6, loads MCM replication helicase complex onto DNA replication origin. GRWD1 has been identified as a novel Cdt1-binding protein. In this research project, we have obtained following findings.(GRWD1 binds to Cdc6 and Cdt1, thereby accumulates onto replication origins.(2) GRWD1is a novel histone-binding protein and has a histone chaperon activity. Furthermore, GRWD1is actually involvedin regulation of chromatin openness(in general, it is believed thatopen chromatin structure promotes various transactions on chromatin).(3) GRWD1 silencing by siRNA inhibits MCM loading. Therefore, GRWD1may play a crucial role in regulation ofreplication.

DNA 复制反应的精确调控对于基因组信息的完整性至关重要。 Cdt1 与 ORC 和 CDC6 一起将 MCM 复制解旋酶复合物加载到 DNA 复制起点上。 GRWD1 已被鉴定为一种新型 Cdt1 结合蛋白。在本研究项目中，我们获得了以下发现：（GRWD1与Cdc6和Cdt1结合，从而积累到复制起点。（2）GRWD1是一种新型组蛋白结合蛋白，具有组蛋白伴侣活性。此外，GRWD1实际上参与染色质的调控开放性（一般认为开放染色质结构促进染色质上的各种交易）。（3）siRNA沉默GRWD1抑制因此，GRWD1可能在复制调节中发挥着至关重要的作用。

项目成果

The Cdt1-binding protein GRWD1 is a novel histone chaperone involved in replication licensing.

Cdt1 结合蛋白 GRWD1 是一种参与复制许可的新型组蛋白伴侣。

• 发表时间: 2011
• 作者: Sugimoto; N.; Yasukouchi; S.; Watanabe; S.; Kiyono; T.; Kurumizaka; H. & Fujita; M.
• 通讯作者: M.

Chromatin Remodeler Sucrose Nonfermenting 2 Homolog (SNF2H) Is Recruited onto DNA Replication Origins through Interaction with Cdc10 Protein-dependent Transcript 1 (Cdt1) and Promotes Pre-replication Complex Formation*

染色质重塑剂蔗糖非发酵 2 同源物 (SNF2H) 通过与 Cdc10 蛋白依赖性转录物 1 (Cdt1) 相互作用被招募到 DNA 复制起点并促进复制前复合物形成*

• DOI: 10.1074/jbc.m111.256123
• 发表时间: 2011-09-20
• 期刊: The Journal of Biological Chemistry
• 作者: Nozomi Sugimoto;T. Yugawa;Masayoshi Iizuka;T. Kiyono;M. Fujita
• 通讯作者: M. Fujita

Redundant and differential regulation of multiple licensing factors ensures prevention of rereplication in normal human cells

多个许可因子的冗余和差异调节确保防止正常人体细胞中的复制

• 发表时间: 2009
• 作者: Sugimoto; N.; Yoshida; K.; Tatsumi; Y.; Yugawa; T.; Narisawa
• 通讯作者: Narisawa

Redundant and differential regulation of multiple licensing factors ensures prevention of rereplication in normal human cells

多个许可因子的冗余和差异调节确保防止正常人体细胞中的复制

• 发表时间: 2009
• 作者: Nozomi Sugimoto

The Cdt1-binding protein GRWD1 is a novel histone chaperone involved in replication licensing

Cdt1 结合蛋白 GRWD1 是一种参与复制许可的新型组蛋白伴侣

• 发表时间: 2011
• 作者: Nozomi Sugimoto; et al
• 通讯作者: et al