Structural Characterization of Heme-mediated Signaling Mechanism in Protein Regulation System

蛋白质调节系统中血红素介导的信号机制的结构表征

• 批准号: 21370040
• 负责人: ISHIMORI Koichiro
• 金额: $ 12.15万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21370040/
• 关键词: ヘム依存性制御蛋白質; 酸化修飾; 活性酸素; ヘム生合成; 鉄代謝

In this research project, we characterized the heme binding in Iron Response Regulator (Irr) and Iron Regulatory Protein (IRP) and examined their functional significance. We spectroscopically identified the heme binding sites of Irr and, based on the structural information, a molecular mechanism of heme-induced oxidative modification in Irr was proposed. The heme binding sites of IRP were also determined and a new mechanism for the translational regulation by binding of heme was suggested in IRP. These new findings in this research project shed new light on the functional significance of heme in vivo.

在这个研究项目中，我们表征了铁反应调节剂 (Irr) 和铁调节蛋白 (IRP) 中的血红素结合，并检查了它们的功能意义。我们通过光谱鉴定了 Irr 的血红素结合位点，并根据结构信息，提出了血红素诱导 Irr 氧化修饰的分子机制。 IRP 的血红素结合位点也被确定，并在 IRP 中提出了通过血红素结合进行翻译调节的新机制。该研究项目的这些新发现为血红素在体内的功能意义提供了新的线索。

项目成果

A Heme Degradation Enzyme, HutZ, fromVibrio cholera

来自霍乱弧菌的血红素降解酶 HutZ

• 发表时间: 2012
• 作者: Uchida; T.; Sekine; Y.; Matsui; T.; Ikeda
• 通讯作者: Ikeda

Oxygen Activation and Oxidative Modification in Heme-regulated Proteins

血红素调节蛋白中的氧活化和氧化修饰

• 发表时间: 2011
• 作者: 石森浩一郎

Two distinct mechanisms of transcriptional regulation by the redox sensor YodB

氧化还原传感器 YodB 的两种不同的转录调节机制

• DOI: 10.1073/pnas.1604427113
• 发表时间: 2016-08-16
• 期刊: Proceedings of the National Academy of Sciences
• 作者: Sang Jae Lee;In;Kiyoung Lee;Dong;Hyun;H. Yoon;Susanna Chae;Sung;Sa‐Ouk Kang;M. Seo;Hyoun Sook Kim;Sung Jean Park;Bong‐Jin Lee
• 通讯作者: Bong‐Jin Lee

Oxidative modification and its functional significance in heme-regulated protein

血红素调节蛋白的氧化修饰及其功能意义

• 发表时间: 2010
• 作者: Ishimori; K.
• 通讯作者: K.

Hepcidin-mediated Iron Regulation in P19 Cells is Detectable by Magnetic Resonance Imaging

磁共振成像可检测 P19 细胞中铁调素介导的铁调节

• DOI: 10.1038/s41598-020-59991-4
• 发表时间: 2020-02-21
• 期刊: Scientific Reports
• 影响因子: 4.6
• 作者: Kobra Alizadeh;Qin Sun;Tabitha McGuire;T. Thompson;F. Prato;J. Koropatnick;N. Gelman;D. Goldhawk
• 通讯作者: D. Goldhawk