Regulatory Mechanisms of Meiosis in Fission Yeast

裂殖酵母减数分裂的调控机制

• 批准号: 21227007
• 负责人: YAMAMOTO Masayuki
• 金额: $ 132.95万
• 依托单位: Kazusa DNA Research Institute (2012-2013)The University of Tokyo (2009-2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (S)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009-05-11 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21227007/
• 关键词: 遺伝学; 発生・分化; 減数分裂; 情報伝達; RNA; 微小管

We investigated the regulatory mechanisms of meiosis in fission yeast. Certain mRNAs required for meiosis are transcribed but undergo degradation called "selective elimination" in vegetatively growing cells. We analyzed this mechanism and identified a number of molecules involved. A pivotal factor Mmi1 protein recognized and bound to specific hexanucleotide motifs on the target mRNAs. meiRNA, which inactivates Mmi1 to promote meiosis, carried numerous copies of the hexanucleotide motifs and lured Mmi1. Meanwhile, we found that phosphorylation of Ser-2 residues on the C-terminal domain (CTD) of RNA polymerase II was essential to transcribe a specific gene required for the initiation of meiosis, and that the stress-responsive MAP kinase pathway was involved in this regulation.

我们研究了裂殖酵母减数分裂的调节机制。减数分裂所需的某些 mRNA 在营养生长细胞中被转录，但会经历称为“选择性消除”的降解。我们分析了这一机制并确定了一些涉及的分子。关键因子 Mmi1 蛋白识别并结合靶 mRNA 上的特定六核苷酸基序。 meiRNA 使 Mmi1 失活以促进减数分裂，它携带大量六核苷酸基序拷贝并引诱 Mmi1。同时，我们发现RNA聚合酶II的C端结构域（CTD）上的Ser-2残基的磷酸化对于转录减数分裂起始所需的特定基因至关重要，并且应激反应性MAP激酶途径参与本规定。

项目成果

Psk1, an AGC kinase family member in fission yeast, is directly phosphorylated and controlled by TORC1 and functions as S6 kinase.

Psk1 是裂殖酵母中的 AGC 激酶家族成员，直接磷酸化并受 TORC1 控制，并起到 S6 激酶的作用。

• DOI: 10.1242/jcs.111146
• 发表时间: 2012
• 影响因子: 4
• 作者: Akio Nakashima

Molecular basis for the selective elimination of meiosis-specific mRNAs in growing cells

选择性消除生长细胞中减数分裂特异性 mRNA 的分子基础

• 发表时间: 2009
• 作者: M. Yamamoto

Molecular basis for the selective elimination of meiosis-specific mRNAs in growing cells.

选择性消除生长细胞中减数分裂特异性 mRNA 的分子基础。

• 发表时间: 2009
• 作者: Yamamoto; Masayuki
• 通讯作者: Masayuki

Molecular basis for the selective elimination of meiosis-specific mRNAs in growing fission yeast cells

在生长的裂殖酵母细胞中选择性消除减数分裂特异性 mRNA 的分子基础

• 发表时间: 2009
• 作者: M. Yamamoto

Meiosis-specific non-coding RNA mediates robust pairing of homologous chromosomes in meiosis

减数分裂特异性非编码RNA介导减数分裂中同源染色体的稳健配对

• 发表时间: 2012
• 影响因子: 56.9
• 作者: D.;Y. Hiraoka
• 通讯作者: Y. Hiraoka