Role of endoplasmic reticulum stress signaling in terminal differentiation of myoblasts

内质网应激信号在成肌细胞终末分化中的作用

• 批准号: 21570210
• 负责人: MORISHIMA Nobuhiro
• 金额: $ 3万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21570210/
• 关键词: 細胞分化; アポトーシス; 筋芽細胞; 小胞体ストレス; カスパーゼ; ATF6; WBP1; Mcl-1; WWドメイン

Overexpression of active ATF6 induces apoptosis in myoblast cells. ATF6 up-regulated WW domain binding protein 1(WBP1), which was found to be proapoptotic. Overexpression of active ATF6 or WBP1 caused a specific decrease in an antiapoptotic protein, myeloid cell leukemia sequence 1(Mcl-1). Cells treated with ER stressors underwent apoptosis and exhibited an up-regulation of WBP1 and decreased Mcl-1.These results suggest that ATF6 mediates apoptosis via specific reduction of Mcl-1 levels through the up-regulation of WBP1.

活性 ATF6 的过度表达会诱导成肌细胞凋亡。 ATF6 上调 WW 结构域结合蛋白 1 (WBP1)，该蛋白被发现具有促凋亡作用。活性 ATF6 或 WBP1 的过度表达导致抗凋亡蛋白、骨髓细胞白血病序列 1 (Mcl-1) 的特异性减少。用 ER 应激源处理的细胞发生凋亡，并表现出 WBP1 上调和 Mcl-1 减少。这些结果表明，ATF6 通过 WBP1 上调特异性降低 Mcl-1 水平来介导细胞凋亡。

项目成果

ATF6 mediates endoplasmic reticulum stress-induced apoptosis with reduction of an anti-apoptotic protein, Mcl-1

ATF6 通过减少抗凋亡蛋白 Mcl-1 介导内质网应激诱导的细胞凋亡

• 发表时间: 2011
• 作者: 森島信裕;中西慶子;中野明彦
• 通讯作者: 中野明彦

Activating Transcription Factor-6 (ATF6) Mediates Apoptosis with Reduction of Myeloid Cell Leukemia Sequence 1 (Mcl-1) Protein via Induction of WW Domain Binding Protein 1*

激活转录因子 6 (ATF6) 通过诱导 WW 结构域结合蛋白 1* 减少骨髓细胞白血病序列 1 (Mcl-1) 蛋白来介导细胞凋亡

• DOI: 10.1074/jbc.m111.233502
• 发表时间: 2011-08-13
• 期刊: The Journal of Biological Chemistry
• 作者: N. Morishima;K. Nakanishi;A. Nakano
• 通讯作者: A. Nakano

Mcl-1 reduction due to caspase-dependent cleavage during endoplasmic reticulum stress-induced apoptosis

内质网应激诱导细胞凋亡过程中半胱天冬酶依赖性裂解导致 Mcl-1 减少

• 发表时间: 2011
• 作者: 森島信裕;中西慶子;中野明彦;Reply to Hu; et al.
• 通讯作者: et al.

Activating transcription factor-6 (ATF6) mediates apoptosis with reduction of myeloid cell leukemia sequence 1 (Mel-1) via induction of WW domain binding protein 1

激活转录因子 6 (ATF6) 通过诱导 WW 结构域结合蛋白 1 减少骨髓细胞白血病序列 1 (Mel-1) 介导细胞凋亡

• DOI: 10.1074/jbc.m111.233502
• 发表时间: 2011
• 作者: Morishima; N.
• 通讯作者: N.

Reply to Hu et al. : Mcl-1 reduction due to caspase-dependent cleavage during endoplasmic reticulum stress-induced apoptosis

回复胡等人。

• DOI: 10.1074/jbc.n111.233502
• 发表时间: 2011
• 作者: Nobuhiro Morishima; Keiko Nakanishi; Akihiko Nakano
• 通讯作者: Akihiko Nakano