Study of the role of two AR subtypes on spiggin synthesis in stickleback kidney

两种AR亚型对刺鱼肾脏spiggin合成的作用研究

• 批准号: 21510069
• 负责人: NAGAE Masaki
• 金额: $ 3.08万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21510069/
• 关键词: バイオマーカー; 環境ホルモン; イトヨ; 男性ホルモン; スピギン; バオマーカー

Full length of ARαand ARβcDNAs were isolated from mature male stickleback kidney. It was showed by reporter gene assay that both were functional AR, and had almost same potency of transcriptional activation. The changes in the mRNA levels of spiggin and both ARs of immature female stickleback treated with androgen were measured and detected by quantitative RT-PCR and in situ hybridization. Spiggin mRNA levels increased in androgen dose and exposure duration-dependent manner. In contrast, the mRNA levels of both ARs were significantly decreased by androgen exposure. In addition, the levels of ARαwere about 10 times higher than that of ARβduring androgen exposure. These results suggested ARαwas essential for the spiggin synthesis in stickleback kidney.

从成熟雄性棘鱼肾脏中分离得到全长的ARα和ARβ cDNA，报告基因检测显示两者均具有功能性AR，并且具有几乎相同的转录激活效力。雌性刺鱼 Spiggin mRNA 水平以雄激素剂量和暴露时间依赖性方式增加，相反，两种 AR 的 mRNA 水平均显着增加。此外，在雄激素暴露期间，ARα的水平比ARβ高约10倍，这些结果表明ARα对于棘鱼肾脏中的spiggin合成至关重要。

项目成果

Effects of ethynylestradiol on vitellogenin synthesis and sex differentiation in juvenile grey mullet(Mugil cephalus) persist after long-term exposure to a clean environment

长期暴露于清洁环境后乙炔雌二醇对幼鱼卵黄原合成和性别分化的影响持续存在

• 发表时间: 2011
• 作者: Aoki J.; Hatsuyama A.; Hiramatsu N.;Soyano K
• 通讯作者: Soyano K

Comparative responsiveness to natural and synthetic estrogens of fish species commonly used in the laboratory and field monitoring

实验室和现场监测中常用的鱼类对天然和合成雌激素的比较反应

• 发表时间: 2012
• 影响因子: 4.5
• 作者: Lange A.; Katsu Y.; Miyagawa S.; Ogino Y.; Urushitani H.; Kobayashi T.; Hirai T.; Shears J. A.; Nagae M.; Yamamoto J.; Ohnishi Y.; Oka T.; Tatarazako N.; Ohta Y.; Tyler C. R.;Iguchi T
• 通讯作者: Iguchi T

The effects of 17beta-estradiol on various reproductive parameters in the hermap hrodite fish Kryptolebias marmoratus.

17β-雌二醇对赫罗迪特鱼 Kryptolebias marmoratus 各种繁殖参数的影响。

• 发表时间: 2010
• 作者: Park CB; Aoki J; Lee JS; Nagae M; Lee YD; Sakakura Y; Hagiwara A; Soyano K
• 通讯作者: Soyano K

The effects of 17beta-estradiol on various reproductive parameters in the hermap hrodite fish Kryptolebias marmoratus.

17β-雌二醇对赫罗迪特鱼 Kryptolebias marmoratus 各种繁殖参数的影响。

• 发表时间: 2010
• 作者: Park CB; Aoki J; Lee JS; Nagae M; Lee YD; Sakakura Y; Hagiwara A; Soyano K
• 通讯作者: Soyano K

使用方法の異なるPPCPsをマーカーとして用いた河川水の生活排水等による汚濁パターンの分類法の開発

以不同使用方法的PPCP为标记，开发河水中生活废水等污染模式的分类方法

• 发表时间: 2011
• 作者: 三浦嘉仁; 山城詩織; 山中潤二; 久保隆; 長江真樹; 有薗幸司; 高尾雄二
• 通讯作者: 高尾雄二