Proteomics of Porphyromonas gingivalis within a model oral microbial community

口腔微生物群落模型中牙龈卟啉单胞菌的蛋白质组学

• 批准号: 20592453
• 负责人: KUBONIWA Masae
• 金额: $ 2.75万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592453/
• 关键词: バイオフイルム; プロテオミクス; 歯周病原性細菌; 代謝経路; ビタミンB; 歯周病; プロテオーム; porphyromonas gingivalis; Fusobacterium nucleatum; Streptococcus gordonii; Porphyromonas gingivalis

P.gingivalis can assemble into a heterotypic community with a middle colonizer F.nucleatum and an early colonizer S.gordonii, and that a community lifestyle provides physiologic support for P.gingivalis. 1156 P.gingivalis proteins, including 403 down-regulated and 89 up-regulated proteins, were detected qualitatively during comparison of the three species model community with P.gingivalis incubated alone under the same conditions. Significant decreases were seen in proteins involved in thiamine, cobalamin, and pyrimidine synthesis. These results indicate that a community lifestyle provides physiologic support for P.gingivalis.

牙龈卟啉单胞菌可以与中间定殖者 F.nucleatum 和早期定殖者 S.gordonii 组装成异型群落，并且群落生活方式为牙龈卟啉单胞菌提供生理支持。将三个物种模型群落与相同条件下单独培养的牙龈卟啉单胞菌进行比较，定性检测到 1156 个牙龈卟啉单胞菌蛋白，其中包括 403 个下调蛋白和 89 个上调蛋白。参与硫胺素、钴胺素和嘧啶合成的蛋白质显着减少。这些结果表明社区生活方式为牙龈卟啉单胞菌提供了生理支持。

项目成果

Identification of binding domain of Streptococcus oralis glyceraldehyde-3-phosphate dehydrogenase for Porphyromonas gingivalis major fimbriae

口腔链球菌甘油醛-3-磷酸脱氢酶与牙龈卟啉单胞菌大菌毛结合域的鉴定

• 发表时间: 2009
• 作者: Nagata; H.; M. Iwasaki; K. Maeda; M. Kuboniwa; E. Hashino; M. Toe; N. Minamino; H. Kuwahara; S. Shizukuishi.
• 通讯作者: S. Shizukuishi.

エリスリトールによる Porphyromonas gingivalis 発現タンパク質の変化に関する網羅的解析

赤藓糖醇引起牙龈卟啉单胞菌表达蛋白变化的综合分析

• 发表时间: 2009
• 作者: 橋野恵衣

Chapter "Heterotypic Streptococcus gordonii-Porphyromonas gingivalis communities : formation, gene regulation and development", in Genomic Inquiries Into Oral Bacterial Communities (ed. Paul E. Kolenbrander)

“异型戈登链球菌-牙龈卟啉单胞菌群落：形成、基因调控和发育”一章，《口腔细菌群落基因组调查》（Paul E. Kolenbrander 编）

• 作者: Kuboniwa; M.
• 通讯作者: M.

Distinct roles of long/short fimbriae and gingipains in homotypic biofilm development by Porphyromonas gingivalis

长/短菌毛和牙龈蛋白酶在牙龈卟啉单胞菌同型生物膜发育中的不同作用

• 发表时间: 2009
• 作者: Kuboniwa; M.
• 通讯作者: M.

Homotypic biofilm structure of Porphyromonas gingivalis is affected by FimA type variations

FimA 类型变异影响牙龈卟啉单胞菌的同型生物膜结构

• 发表时间: 2009
• 作者: Kuboniwa; M.
• 通讯作者: M.