The analysis and significance of the autoderived-growthfactor as a stimulator of the proliferation and the differentiation of human dental pulp stem cells

自体生长因子对人牙髓干细胞增殖分化刺激作用的分析及意义

• 批准号: 20592232
• 负责人: KATAYAMA Tadashi
• 金额: $ 3万
• 依托单位: Meikai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592232/
• 关键词: 再生医学; 歯学; 歯髄; 生体材料; PRP

The efficacy of the extract of washed platelet (WPLT) as a stimulator of the proliferation and the calcification of human dental pulp stem cells was investigated. The growth of pulp cells was weakly stimulated by treatment with platelet-rich plasma (PRP), while it was more effectively stimulated by WPLT. Moreover, platelet-poor plasma (PPP) dose-dependently inhibited the growth of cultured human dental pulp cells. WPLT stimulated the calcification induced by β-glycerophosphate and ascorbic acid in the pulp cells, although WPLT alone was ineffective. WPLT significantly stimulated the mRNA expression of bone Gla protein (BGP), but not that of bone sialoprotein (BSP) or dentin sialoprotein (DSP), β-glycerophosphate and ascorbic acid stimulated both BGP and DSP, and addition of WPLT with BGP and Aa did not produce any addition or ayregic effect of the mRNA expression by β-glycerophosphate and ascorbic acid. These results suggest that WPLT is a potent stimulator of human dental pulp cells, and play a significant role in bone calcification.

研究了洗涤血小板提取物 (WPLT) 作为人牙髓干细胞增殖和钙化刺激剂的功效。此外，贫血小板血浆（PPP）可以剂量依赖性地抑制培养的人牙髓细胞的生长，并刺激β-甘油磷酸诱导的钙化。牙髓细胞中的抗坏血酸，虽然单独的WPLT不能显着刺激骨Gla蛋白（BGP）的mRNA表达，但不能刺激骨唾液蛋白（BSP）或牙本质唾液蛋白（DSP）、β-甘油磷酸和抗坏血酸。 BGP 和 DSP 均有效，并且添加 WPLT 与 BGP 和 Aa 不会对 mRNA 表达产生任何附加或不规则效应这些结果表明 WPLT 是人牙髓细胞的有效刺激剂，并且在骨钙化中发挥重要作用。

项目成果

4-トリフルオロメチルイミダゾール誘導体の腫瘍選択性と誘導される細胞死のタイプ

4-三氟甲基咪唑衍生物诱导的肿瘤选择性和细胞死亡类型

• 作者: 竹川文弘;岸野香織;天野修;菊地寛高;石原真理子;坂上宏
• 通讯作者: 坂上宏

培養ヒト歯肉線維芽細胞の増殖に及ぼすCO_2レーザー照射のホルメシス効果

CO_2激光照射对培养人牙龈成纤维细胞增殖的毒物兴奋效应

• 作者: 岩坂憲助;安永慎;山田晶子;段谷由香;坂上宏;片山直
• 通讯作者: 片山直

Podoplanin expression in odontomas : clinicopathological study and immunohistochemical analysis of 86 case

牙瘤中Podoplanin的表达：86例临床病理学研究及免疫组化分析

• 发表时间: 2010
• 作者: Gonzalez

Podoplanin expression in odontomas : clinicopathological study and immunohistochemical analysis of 86 case

牙瘤中Podoplanin的表达：86例临床病理学研究及免疫组化分析

• 发表时间: 2010
• 影响因子: 1.9
• 作者: Gonzalez

培養ヒト歯肉線維芽細胞の増殖に及ぼすCO2レーザー照射のホルメシス効果

CO2激光照射对培养人牙龈成纤维细胞增殖的兴奋效应

• 发表时间: 2009
• 作者: 岩坂憲助; 安永慎; 山田晶子; 段谷由香; 坂上宏; 片山直
• 通讯作者: 片山直