Homing of circulating osteogenic progenitors to sites of fracture repair under the low-intensity pulsed ultrasound treatment

低强度脉冲超声治疗下循环成骨祖细胞归巢至骨折修复部位

• 批准号: 20591763
• 负责人: SAITO Tomoyuki
• 金额: $ 3.24万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591763/
• 关键词: 骨折治癒; 低出力超音波; 末梢血由来細胞; ホーミング; 骨形成促進効果; 骨形成促進

A parabiotic model was created by surgically conjoining one transgenic GFP mouse and one wild-type partner. After shared circulation was established, a transverse fracture with periosteal stripping was created in the contralateral diaphysial femur of the wild-type partner. The fracture site was treated with daily exposure of low-intensity pulsed ultrasound (LIPUS). GFP+ cells that gained access to the osteotomy site via the systemic circulation were identified in the bone-forming site. The findings suggested that LIPUS induced homing of circulating osteogenic progenitors to the osteotomy site for possible contribution to new bone formation.

通过手术将一只转基因 GFP 小鼠和一只野生型伙伴连接起来，创建了一种联体生物模型。建立共享循环后，在野生型伴侣的对侧股骨干处产生骨膜剥离的横向骨折。骨折部位每天接受低强度脉冲超声（LIPUS）照射治疗。在骨形成部位鉴定出通过体循环进入截骨部位的 GFP+ 细胞。研究结果表明，LIPUS 诱导循环成骨祖细胞归巢至截骨部位，可能有助于新骨形成。

项目成果

Relationship between low bone mineral density and varus deformity in postmenopausal women with knee osteoarthritis.

绝经后膝骨关节炎女性低骨密度与内翻畸形的关系。

• 发表时间: 2009
• 作者: Akamatsu Y; Mitsugi N; Taki N; Takeuchi R; Saito T.
• 通讯作者: Saito T.

Low-intensity pulsed ultrasound stimulates iIntramedullary bone formation and homing of circulating connective tissue progenitors at fracture site with periosteal stripping in mice.

低强度脉冲超声通过小鼠骨膜剥离刺激骨折部位的髓内骨形成和循环结缔组织祖细胞的归巢。

• 发表时间: 2009
• 作者: 熊谷研; 竹内良平; 荒武正人; 斎藤泉; 尾藤晴彦; 佐々木洋平; 齋藤知行
• 通讯作者: 齋藤知行

A mid term comparison of open wedge high tibial osteotomy vs unicompartmental knee arthroplasty for medial compartment osteoarthritis of the knee.

开放楔形胫骨高位截骨术与单间室膝关节置换术治疗膝关节内侧间室骨关节炎的中期比较。

• 发表时间: 2010
• 作者: Takeuchi R; Umemoto Y; Aratake M; Bito H; Saito I; Kumagai K; Sasaki Y; Akamatsu Y; Ishikawa H; Koshino T; Saito T.
• 通讯作者: Saito T.

Opening wedge high tibial osteotomy affects both the lateral patellar tilt and patellar height.

胫骨高位切开楔形截骨术会影响髌骨外侧倾斜和髌骨高度。

• 发表时间: 2010
• 作者: Bito H; Takeuchi R; Kumagai K; Aratake M; Saito I; Hayashi R; Sasaki Y; Saito T.
• 通讯作者: Saito T.

Clinical results and radiographical evaluation of opening wedge high tibial osteotomy for spontaneous osteonecrosis of the knee.

开放式胫骨高位楔形截骨术治疗膝关节自发性骨坏死的临床结果和影像学评估。

• 发表时间: 2009
• 作者: Takeuchi R; Aratake M; Bito H; Saito I; Kumagai K; Hayashi R; Sasaki Y; Akamatsu Y; Ishikawa H; Amakado E; Aota Y; Saito T.
• 通讯作者: Saito T.