Development of new dental pulp therapy using tooth bud organ culture technique.

利用牙芽器官培养技术开发新的牙髓疗法。

基本信息

批准号：
20791393
负责人：
NAKA Takahiro
金额：
$ 1.16万
依托单位：
Ohu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2009
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791393/
关键词：
歯髄組織組織培養再生療法物理刺激骨代謝歯科用インプラント骨細胞 SOST遺伝子オッセオインテグレーション形態学的解析発生形態形成唾液腺(顎下腺・舌下腺)口腔上皮間葉相互作用 Homeobox遺伝子

项目摘要

(1) The development of mouse submandibular gland (SMG) begins at embryonic day 11.5-12 (E11.5-12), during which successive rounds of epithelial clefting and branching create complex epithelial tree-like structures. Homeobox genes regulate place-dependent morphogenesis, including epithelial-mesenchymal interactions, and control the expression patterns of signaling molecules. The Barx2 containing Homeobox exerts several key roles in development. The results of this study indicate that Barx2 is associated with early stage epithelial tissue development, and can be a useful epithelial marker of the SMG during early developmental stages.(2) Carbon dioxide laser (CO_2 laser) is common laser used in daily dental clinic. But the application of the CO_2 laser is restricted to the treatment of the soft tissue. This study evaluated the application of laser-induced bone therapy (LIBT) using CO_2 laser to reduce implant healing time in rat tibia. Forty-two 10-week-old female Sprague-Dawlay (SD) rats … More were used in this study. The animals were randomly divided into control (Group A) and experimental groups (Group B). The rats in both groups received laser irradiation or bur injury on either side of the tibia. Five days after invasion, titanium implants were inserted in proximal tibia of the experimental group rats. Five, 10, and 20 days after implant placement, the animals were sacrificed and tibiae were collected. After taking soft X-ray images and performing a torque test, the tibias were decalcified and 8-μm-thick sections were prepared. Specimens were stained with hematoxylin and eosin. Soft-X ray images, removal torque values, and histomorphometric analysis data demonstrated a significantly accelerated bone formation in the laser-implant group earlier in the healing process. Until 10 days, this phenomenon was continuously observed. Twenty days after implant placement, comparable results were obtained in both the laser-implant and bur-implant groups. I conclusion, the use of laser irradiation prior to implantation was effective in promoting bone formation and acquiring osseointegration of titanium implants inserted in rat tibia. LIBT may be suitable for use in oral implant therapy. Less

(1) 小鼠颌下腺 (SMG) 的发育开始于胚胎第 11.5-12 天 (E11.5-12)，在此期间连续几轮的上皮分裂和分支形成复杂的上皮树状结构。形态发生，包括上皮-间质相互作用，并控制信号分子的表达模式。含有同源盒的 Barx2 在发育中发挥着几个关键作用。本研究结果表明Barx2与早期上皮组织发育相关，可以作为SMG早期发育阶段有用的上皮标志物。（2）二氧化碳激光（CO_2激光）是日常使用的常用激光牙科诊所但CO_2激光的应用仅限于软组织的治疗。本研究评估了使用CO_2激光的激光诱导骨治疗（LIBT）在大鼠胫骨中缩短种植体愈合时间的应用。本研究使用10周龄雌性Sprague-Dawlay（SD）大鼠，将动物随机分为对照组（A组）和实验组（B组），两组大鼠均接受激光照射或车针照射。侵入后五天，将钛植入物植入实验组大鼠的胫骨近端，植入物植入后五天、十天和二十天，对动物进行植入。采集软X射线图像并进行扭矩测试后，将胫骨脱钙并制备8μm厚的切片，用苏木精和伊红染色，去除扭矩值。，组织形态分析数据表明，激光种植组在愈合过程的早期骨形成明显加速，直到种植体植入后 20 天，才持续观察到这种现象。在激光植入组和钻头植入组中均获得了结果，结论是，植入前使用激光照射可有效促进骨形成，并获得插入大鼠胫骨的钛植入物的骨整合。口腔种植治疗较少。