Relationship between administration of molecular hydrogen and stroke

分子氢给药与中风之间的关系

• 批准号: 20790628
• 负责人: NISHIYAMA Yasuhiro
• 金额: $ 1.25万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790628/
• 关键词: 脳卒中; 水素水; 水素ガス; 脳梗塞

Clinical evidence as well as experimental results strongly suggests the major contribution of oxidative stress to stroke. The recent study reported that molecular hydrogen (H2) reduces oxidative stress and limits infarct volume of the brain. Moreover, the other study showed that the consumption of water with dissolved H2 to a saturated level prevents stress nigrostriatal degeneration. A variety of H2 delivery systems are used for effective administration. I investigate whether this simple method to deliver H2 into body or organs has the potential to prevent stroke. I tried two preliminary experiments. First, a separate population of 8-week-old SHRSPs was divided into two groups of 18 animals each and one group administered H2 water, which is replaced each day. Mean survival day did not differ significantly between two groups (DW group 107.5±38.6 days vs H2 group 105.85±39.5 days). This study suggested that giving hydrogen water to SHRSP rats did not influence the survival time. Second, rats were subjected to transient focal ischemia for 90 min by the intraluminal suture technique. Rats received intravenous injections of 5.0mg/kg of hydrogen dissolved in saline water or saline. Hydrogen water degassed by gentle stirring was used for control animals. Rats were ramdomly divided into three groups of 5 animals each: (I) 5 min before reperfusion group; (II) 60 min after reperfusion group, (III) saline group (control). But there is no significant difference in the infarction volume among three groups. This study suggested that injection of hydrogen dissolved in saline may have the potential effect on ischemic stroke, but further study is required to investigate how the timing or the route of administration of H2 is appropriate.

临床证据和实验结果强烈表明氧化应激对中风有重要影响。最近的研究报告称，氢分子 (H2) 可以减少氧化应激并限制大脑的梗塞体积。此外，另一项研究表明，水的消耗也可以减少脑梗塞的发生。溶解的氢气达到饱和水平可防止应激性黑质纹状体变性。我尝试了两种方法来研究这种将氢气输送到体内或器官的简单方法是否有可能预防中风。首先，将 8 周龄的 SHRSP 群体分为两组，每组 18 只，其中一组给予 H2 水，每天更换一次，两组之间的平均存活天数没有显着差异。 107.5±38.6 天 vs H2 组 105.85±39.5 天）这项研究表明，给 SHRSP 大鼠喂食氢水不会影响存活时间。采用腔内缝合技术将大鼠静脉注射5.0mg/kg的氢气或轻轻搅拌脱气的氢气水作为对照动物，将大鼠随机分为三组，每组5只。各：（I）再灌注前5分钟组；（II）再灌注后60分钟组；（III）盐水组（对照）但没有显着差异。本研究表明，注射溶解在盐水中的氢气可能对缺血性中风有潜在影响，但需要进一步研究以探讨注射氢气的时间或途径是否合适。