Analysis of structural elements controlling transglycosylation and its application

控制转糖基化的结构元件分析及其应用

• 批准号: 20380056
• 负责人: KIMURA Atsuo
• 金额: $ 12.4万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20380056/
• 关键词: 糖転移; 構造因子; 分子解析

Glycosylases are enzymes, which are currently utilized for the production of oligosaccharides. Interestingly, production-ability is dependent on the kinds of glycosylases, meaning that their structural elements contribute to the reaction specificity. Our research aims at elucidating structural elements of glycosylase-catalyzed transglycosylation. Our research achieved i) elucidation of structural elements contributing to the oligosaccharide production ; ii) establishment of the theoretical background of oligosaccharide production ; iii) finding the ways to regulate the oligosaccharide production.

糖基酶是目前用于生产寡糖的酶。有趣的是，生产能力取决于糖基酶的种类，这意味着它们的结构元件有助于反应特异性。我们的研究旨在阐明糖基酶催化的转糖基化的结构元件。我们的研究成果 i) 阐明了有助于寡糖生产的结构元件； ii) 建立低聚糖生产的理论背景； iii) 寻找调节寡糖生产的方法。

项目成果

Bioengineered glucansucrase of Leuconostoc mesenteroides for preferred oligosaccharide synthesis using sucrose

肠膜明串珠菌的生物工程葡聚糖蔗糖酶，用于使用蔗糖合成优选的寡糖

• 发表时间: 2011
• 作者: Kang HK; Kimura A; Kim D
• 通讯作者: Kim D

Truncation of N- and C-terminal regions of Streptococcus mutans dextranase enhances catalytic activity

变形链球菌葡聚糖酶 N 和 C 末端区域的截短增强了催化活性

• 发表时间: 2011
• 作者: Kim YM; Shimizu R; Nakai H; Mori H; Okuyama M; Kang MS; Fujimoto Z; Funane K; Kim D; Kimura A
• 通讯作者: Kimura A

Rice Os4BGlu12 is a wound-induced glucosidase that hydrolyzes cell-wall-glucan-derived oligosaccharides and glycosides

Rice Os4BGlu12 是一种伤口诱导的<【○！R】->葡萄糖苷酶，可水解细胞壁-<【○！R】->葡聚糖衍生的寡糖和糖苷

• 发表时间: 2010
• 影响因子: 5.2
• 作者: Opassiri R

Construction of a fusion enzyme of dextransucrase and dextranase : Application for one-step synthesis of isomaltooligosaccharides.

葡聚糖蔗糖酶和葡聚糖酶融合酶的构建：用于一步合成低聚异麦芽糖的应用。

• 发表时间: 2009
• 作者: Kim YM; Seo MY; Kang HK; Kimura A; Kim D
• 通讯作者: Kim D

Isolation of Bacillus and Paenibacillus bacterial strains that produce large molecular of cyclic isomaltooligosaccharides.

产生大分子环状低聚异麦芽糖的芽孢杆菌和类芽孢杆菌菌株的分离。

• 发表时间: 2008
• 作者: Funane K; Terasawa K; Mizuno Y; Ono H; Gibu S; Tokashiki T; Kawabata Y; Kim YM; Kimura A; Kobayashi M
• 通讯作者: Kobayashi M