ウラギンシジミにおける視覚的種内他個体認識の神経行動学的研究

乌拉吉纳塔其他个体视觉种内识别的神经行为研究

基本信息

批准号：
14F04764
负责人：
蟻川謙太郎
金额：
$ 1.47万
依托单位：
The Graduate University for Advanced Studies
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2014
资助国家：
日本
起止时间：
2014-04-25 至 2017-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14F04764/
关键词：
insect vision angled sunbeam butterfly intraspecific signaling retinal histology eyeshine spectral sensitivity spatial sensitivity LED stimulation retinal hisgology angled sunbeam visual signalling laboratory rearing

项目摘要

A major advance this year was implemented in the telemicroscope setup, which has a spectrophotometer head and a side port. Two images from different focal planes and a spectrum from a defined region can be recorded simultaneously. We used the setup for hyperspectral imaging of eye reflectance and fluorescence, corneal/ tissue slide transmittance and for rhodopsin dynamics. The spectroscopy and dynamic pupil activation experiments on Curetis acuta revealed that the long wavelength rhodopsin peaks at 575 nm. Furthermore, by analyzing the pupils of individual ommatidia to spectral stimulation, we found five to six clusters of ommatidial types, consistent with three short-wavelength receptor classes. The method is certainly a promising tool for studying butterfly eyes with red eyeshine. We thus applied the method to several lepidopteran species including a skipper and two hawkmoths.Another advance was in building light stimulation device using LEDs. We developed an image analysis method using the device for estimating pigment concentrations in a hyperspectral image stack. To validate the device as a biomedical tool, we measured blood oxygenation in the frog skin: Dr Pirih published the results (Belusic et al. Sci rep, 6:32012, 2016). We now have a two-register LED synthesizer with anamorphic optics and a depolarizer in Sokendai, which Dr Pirih assembled successfully during his extended stay for 2 months. This will be a powerful research tool in my laboratory in coming years.

今年的远程镜设置实施了重大进步，该设置具有分光光度计头和侧端口。可以同时记录来自不同焦点平面的两个图像和来自定义区域的光谱。我们将设置用于眼部反射率和荧光，角膜/组织滑动透射率以及视蓝蛋白动力学的高光谱成像。 curetis acuta上的光谱和动态瞳孔激活实验表明，长波长视紫红质峰在575 nm处。此外，通过分析单个果皮刺激的学生的学生，我们发现了五到六个膜类型的簇，与三个短波长受体类别一致。该方法无疑是一种有前途的工具，用于研究红眼睛的眼睛。因此，我们将该方法应用于几种鳞翅目物种，包括船长和两个霍克莫斯。另一个前进是使用LED构建光刺激装置。我们使用该设备开发了一种图像分析方法来估计高光谱图像堆栈中的色素浓度。为了验证该设备作为生物医学工具，我们测量了青蛙皮肤中的血液氧合：Pirih博士发表了结果（Belusic等人SCI REP，6：32012，2016）。现在，我们拥有一个两次注册的LED合成器，该合成器具有变形光学元件和一个在Sokendai的depolarizer，Pirih博士在长时间的住宿期间成功组装了2个月。这将是未来几年我实验室中的强大研究工具。