Plant Metabolism of Xenobiotics

外源物质的植物代谢

• 批准号: 5428147
• 负责人: Professor Dr. Erwin Grill
• 金额: --
• 依托单位: Lehrstuhl für Botanik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2004
• 资助国家: 德国
• 起止时间: 2003-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5428147?language=en
• 关键词: Plant; Metabolism; Xenobiotics

Phytochelatin synthase not only catalyzes the heavy metal-dependent synthesis of phytochelatins but also serves as a catalyst for the key step in the turnover of lutathione conjugates in plants, the formation of g-glutamyl-cysteinyl conjugates. The latter function has recently been assigned by in vitro analyses. The project proposes now to test and corroborate the newly identified function of PCS in vivo. The analysis will focus on Arabidopsis by using plants, protoplasts and plant cell cultures. Mutants of Arabidopsis impaired in or lacking PCS will be analyzed for glutathione-conjugate turnover and phenotypic alterations. In addition, it is planned to transiently modulate phytochelatin synthase gene expression in order to assess its functions. The cellular compartmentation of both the reaction and of the reaction product will be examined including transport studies of the generated glutathioneconjugate derivative with isolated vacuoles.

植物螯合素合酶不仅催化重金属依赖性植物螯合素合成，而且还充当植物中谷胱甘肽缀合物周转的关键步骤（g-谷氨酰-半胱氨酰缀合物形成）的催化剂。最近通过体外分析确定了后一种功能。该项目现在建议测试并证实新发现的 PCS 体内功能。该分析将通过使用植物、原生质体和植物细胞培养物来重点研究拟南芥。将分析 PCS 受损或缺乏的拟南芥突变体的谷胱甘肽缀合物周转和表型改变。此外，计划瞬时调节植物螯合素合酶基因表达以评估其功能。将检查反应和反应产物的细胞区室，包括产生的谷胱甘肽缀合物衍生物与分离液泡的转运研究。