Development of growth inhibition technology of toxic-producing blue-green algae

产毒蓝绿藻生长抑制技术开发

• 批准号: 19760370
• 负责人: HIDAKA Taira
• 金额: $ 2万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19760370/
• 关键词: 水質汚濁・土壌汚染防止・浄化; モデル化; 有害化学物質; 有害物質生成藻類; Microcystis属; 培養実験; リアルタイムPCR; 遺伝子; 検鏡; 水質; Microcystin

Applicability of real-time PCR method was evaluated by cultivation experiments of Microcystis. After logarithmic growth phase, quantification results by both mcy A gene and 16S rRNA gene were similar to cell numbers measured by microscopic analysis. Increase in cell number and mcy A gene corresponded with increase in microcystin production. Simultaneous increase in mcy A gene and microcystin was observed in a field survey. These results proved that real-time PCR quantification can be applied to microcystin-producing blue-green algae, and it is promising to develop monitoring and growth inhibition technologies of toxic-producing blue-green algae.

通过微囊藻培养实验评价实时PCR方法的适用性。对数生长期后，mcy A基因和16S rRNA基因的定量结果与显微镜分析测量的细胞数相似。细胞数量和 mcy A 基因的增加与微囊藻毒素产量的增加相对应。现场调查观察到mcy A基因和微囊藻毒素同时增加。这些结果证明实时PCR定量可以应用于产生微囊藻毒素的蓝绿藻，并且有希望开发产生毒素的蓝绿藻的监测和生长抑制技术。

项目成果

Analysis of factors affecting the ratio of microcystin to chlorophyll-a in cyanobacterial blooms using real-time polymerase chain reaction

利用实时聚合酶链反应分析蓝藻水华中微囊藻毒素与叶绿素-a 比例的影响因素

• 作者: Joo H. Ha; Taira Hidaka; Hiroshi Tsuno
• 通讯作者: Hiroshi Tsuno

リアルタイムPCRによるミクロシスチン生成藍藻類の分析

通过实时 PCR 分析产生微囊藻毒素的蓝藻

• 发表时间: 2009
• 作者: 日高平; 河周賢; 楠田育成; 津野洋
• 通讯作者: 津野洋