Dorsal and ventral specification in optic cup derived from ES cells in vitro

体外 ES 细胞衍生视杯的背侧和腹侧规格

• 批准号: 24680037
• 负责人: EIRAKU Mototsugu
• 金额: $ 4.66万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24680037/
• 关键词: パターン形成; 自己組織化; 幹細胞; 組織の三次元形態; 極性; ES細胞

In this study, we aimed to elucidate the molecular mechanisms underlying the formation of the dorsal-ventral polarity in the optic cup-like structure derived from ES cells. Dorsal and ventral specification in the optic cup appears to play an important role in the proper development of the eye. But, it remains elusive how the dorsal-ventral polarity is established in the optic vesicle. We found that ventral and dorsal markers expressed in a mutually exclusive pattern in the neural retina derived from ES cells. This result indicates that retinal neuroepithelium can spontaneously acquire the dorsal-ventral polarity. By time-lapse imaging study of Wnt signaling dynamics and pharmacological experiments, it is revealed that the dorsal and ventral specification of the optic cup is regulated by spatially and temporary controled molecular signals including Wnt, BMP and Shh signaling.

在这项研究中，我们旨在阐明在源自ES细胞的视觉杯状结构中形成背腹极性的基础机制。视杯中的背侧和腹侧规格似乎在眼睛的正常发育中起着重要作用。但是，在视囊中如何建立背腹极是如何建立的，仍然难以捉摸。我们发现，在源自ES细胞的神经视网膜中以相互排斥的模式表达的腹侧和背侧标记。该结果表明视网膜神经上皮可以自发地获得背腹极性。通过对Wnt信号动力学和药理学实验的延时成像研究，揭示了视觉杯的背侧和腹侧规范受空间和临时控制的分子信号（包括Wnt，BMP和SHH信号传导）调节。

项目成果

Self-organized formation of optic cup in vitro Mototsugu Eiraku Joint

体外视杯自组织形成 Mototsugu Eiraku Joint

• 发表时间: 2012

多能性幹細胞からの立体組織形成

多能干细胞的三维组织形成

• 发表时间: 2014
• 作者: Hase K;Fujiwara Y;Kikuchi H;Aizawa S;Hakuno F;Takahashi S;Wada K;Kabuta T.;永楽 元次
• 通讯作者: 永楽 元次

Reversible network reconnection model for simulating large deformation in dynamic tissue morphogenesis

• DOI: 10.1007/s10237-012-0430-7
• 发表时间: 2013-08-01
• 期刊: BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
• 影响因子: 3.5
• 作者: Okuda, Satoru;Inoue, Yasuhiro;Adachi, Taiji
• 通讯作者: Adachi, Taiji

Modeling cell proliferation for simulating three-dimensional tissue morphogenesis based on a reversible network reconnection framework

• DOI: 10.1007/s10237-012-0458-8
• 发表时间: 2013-10-01
• 期刊: BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
• 影响因子: 3.5
• 作者: Okuda, Satoru;Inoue, Yasuhiro;Adachi, Taiji
• 通讯作者: Adachi, Taiji

in vitroでの立体網膜組織の自己形成とその原理

三维视网膜组织的体外自形成及其原理

• 发表时间: 2013
• 作者: Tao;S.;Yamazaki;D.;Komazaki;S.;Zhao;C.;Iida;T.;Kakizawa;S.;Imaizumi;Y. & Takeshima;H.;永楽 元次
• 通讯作者: 永楽 元次