Identification and characterization of Vpx-interacting host factors

Vpx 相互作用宿主因子的识别和表征

• 批准号: 24790447
• 负责人: MIYAKAWA Kei
• 金额: $ 2.91万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24790447/
• 关键词: 翻訳後修飾; 免疫応答

HIV-2/SIVsm Vpx promotes the viral replication through the degradation of a host antiviral factor, SAMHD1, in myeloid cells. Although it has been reported that phosphorylation status of SAMHD1 was important for its antiviral activity, it is still unknown whether the phosphorylation of Vpx contributes its anti-SAMHD1 activity. In this study we identified several human kinases as potential host factors to interact with Vpx. Further biochemical analysis uncovered that one kinase directly interacted with Vpx and promoted its phosphorylation. This phosphorylation is found to be important on the degradation of SAMHD1 as well as efficient viral infection. Our results together suggest that a kinase we identified could regulate the Vpx-dependent viral replication.

HIV-2/SIVSM VPX通过在髓样细胞中的宿主抗病毒因子SAMHD1的降解来促进病毒复制。尽管据报道，SAMHD1的磷酸化状态对于其抗病毒活性很重要，但VPX的磷酸化是否促进其抗SAMHD1活性，仍然尚不清楚。在这项研究中，我们确定了几种人类激酶是与VPX相互作用的潜在宿主因素。进一步的生化分析发现，一种激酶直接与VPX相互作用并促进其磷酸化。发现这种磷酸化对于SAMHD1的降解以及有效的病毒感染至关重要。我们共同的结果表明，我们确定的激酶可以调节依赖VPX的病毒复制。