Analysis of body axis formation of mouse postimplantation development

小鼠着床后发育体轴形成分析

• 批准号: 24770219
• 负责人: SHIOI Go
• 金额: $ 3万
• 依托单位: Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24770219/
• 关键词: 前後軸形成; 胚培養; ライブイメージング; レポーターマウス; 細胞挙動; 全胚培養

To reveal the mechanism of the anterior-posterior (A-P) axis formation in a mouse embryo, the expression pattern of head organizer genes have been reexamined. I found that OTX2 gene was asymmetrically expressed from the very beginning of their expression. The gene which makes Otx2 express asymmetrically might have an important role to develop A-P axis. I also established a culture system of mouse embryos for live-imaging experiments. Then, the behavior of each embryonic visceral endoderm cell during the A-P axis formation has been examined by live-imaging experiments. I found some characteristic cell behaviors which remained to be reported.

为了揭示小鼠胚胎中前后（A-P）轴形成的机制，我们重新检查了头部组织者基因的表达模式。我发现OTX2基因从一开始表达就是不对称的。使Otx2不对称表达的基因可能在A-P轴的发育中起重要作用。我还建立了用于实时成像实验的小鼠胚胎培养系统。然后，通过实时成像实验检查了每个胚胎内脏内胚层细胞在 A-P 轴形成过程中的行为。我发现了一些有待报告的特征性细胞行为。

项目成果

Live Imaging Analysis of Mouse Development during AVE Formation

AVE 形成过程中小鼠发育的实时成像分析

• 发表时间: 2014
• 作者: Go Shioi;Hideharu Hoshino;Kana Bando;Kazuki Nakao;Toshihiko Fujimori and Shinichi Aizawa
• 通讯作者: Toshihiko Fujimori and Shinichi Aizawa

Visualization of cell cycle in mouse embryos with Fucci2 reporter directed by Rosa26 promoter

• DOI: 10.1242/dev.084111
• 发表时间: 2013-01-01
• 期刊: DEVELOPMENT
• 影响因子: 4.6
• 作者: Abe, Takaya;Sakaue-Sawano, Asako;Fujimori, Toshihiko
• 通讯作者: Fujimori, Toshihiko

OTX2 and DKK1 Exhibit the Earliest Asymmetric Expression in Distal Visceral Endoderm.

OTX2 和 DKK1 在远端内胚层中表现出最早的不对称表达。

• 发表时间: 2014
• 作者: Hideharu Hoshino;Go Shioi;Hiroshi Kiyonari;Takaya Abe;Kazuki Nakao;Shinichi Aizawa
• 通讯作者: Shinichi Aizawa

Reporter Mouse Lines for Live Imaging

用于实时成像的记者鼠标线

• 发表时间: 2013
• 作者: Takaya Abe;Hiroshi Kiyonari;Go Shioi;Kenichi Inoue;Kazuki Nakao;Shinichi Aizawa;Yasuhide Furuta and Toshihiko Fujimori
• 通讯作者: Yasuhide Furuta and Toshihiko Fujimori

Live imaging of pre-implantation mouse embryos labeld with fluorescent fusion proteins

用荧光融合蛋白标记的植入前小鼠胚胎的实时成像

• 发表时间: 2012
• 作者: Hiroshi Kiyonari;Takaya Abe;Mari Kaneko;Go Shioi;Kazuki Nakao;Shinichi Aizawa;Toshihiko Fujimori
• 通讯作者: Toshihiko Fujimori