Molecula mechanism of mRNA targeting to the ER in SRP-independent manner

mRNA以不依赖SRP的方式靶向ER的分子机制

• 批准号: 24770185
• 负责人: YOSHIDA Hideki
• 金额: $ 2.91万
• 依托单位: Kyoto Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24770185/
• 关键词: RNAイメージング; mRNAの小胞体標的化; ショウジョウバエ; 培養細胞; mRNAの局在化; 小胞体標的化; mRNA

To reveal the novel mechanism of mRNA targeting to the ER, we had established green fluorescent protein (GFP)-mediated RNA visualization system in Drosophila cultured cells to analyze the mRNA localization and widely identified the mRNAs which are targeted to the ER in a novel manner. As a result of the visualization of mRNA localization, which is targeted in a conventional mechanism by using the system we established, GFP dot signals were observed in the cytoplasm. On the other hand, the GFP signals were hardly observed in the conventional targeting mechanism-disrupted cells. From these data, we have judged the visualization system we established certainly works. By this visualization system, the localization of mRNA, which was identified as a mRNA targeted to the ER in non-conventional manner, was observed and the mRNA also formed foci in the cytoplasm. Interestingly, the GFP dot formation was not affected even if the conventional mechanism was suppressed by RNA interference.

为了揭示mRNA靶向内质网的新机制，我们在果蝇培养细胞中建立了绿色荧光蛋白（GFP）介导的RNA可视化系统来分析mRNA定位，并以新的方式广泛鉴定靶向内质网的mRNA 。通过使用我们建立的系统以常规机制靶向mRNA定位的可视化，结果在细胞质中观察到了GFP点信号。另一方面，在常规靶向机制被破坏的细胞中几乎观察不到 GFP 信号。从这些数据来看，我们建立的可视化系统确实有效。通过该可视化系统，观察到mRNA的定位，该mRNA被鉴定为以非常规方式靶向ER的mRNA，并且该mRNA也在细胞质中形成了焦点。有趣的是，即使RNA干扰抑制了常规机制，GFP点的形成也没有受到影响。

项目成果

mRNA新規小胞体標的化機構の分子メカニズムの解明

阐明mRNA新型内质网靶向机制的分子机制

• 发表时间: 2012
• 作者: 梅河内隆成;臼井一馬;佐藤哲也;須山幹太;大川恭行;山口政光;吉田英樹
• 通讯作者: 吉田英樹

RNA-based diagnosis in a multicellular specimen by whole mount in situ hybridization using an RNA-specific probe.

• DOI: 10.1016/j.bmc.2012.08.028
• 发表时间: 2012-10
• 期刊: Bioorganic & medicinal chemistry
• 影响因子: 3.5
• 作者: T. Ueda;A. Kobori;A. Yamayoshi;H. Yoshida;M. Yamaguchi;A. Murakami

ショウジョウバエDNAポリメラーゼp255サブユニットと相互作用する遺伝子の探索

寻找与果蝇 DNA 聚合酶 p255 亚基相互作用的基因

• 发表时间: 2012
• 作者: Ruan K;Yamamoto TG;Asakawa H;Chikashige Y;Haraguchi T;Masukata H;Hiraoka Y;島村真衣;前田頌太
• 通讯作者: 前田頌太

Drosophila myeloid leukemia factor acts with DREF to activate the JNK signaling pathway.

• DOI: 10.1038/oncsis.2014.13
• 发表时间: 2014-04-21
• 期刊: ONCOGENESIS
• 影响因子: 6.2
• 作者: Yanai, H.;Yoshioka, Y.;Yoshida, H.;Nakao, Y.;Plessis, A.;Yamaguchi, M.
• 通讯作者: Yamaguchi, M.

ショウジョウバエDOCK family遺伝子の機能解析

果蝇DOCK家族基因的功能分析

• 发表时间: 2012
• 作者: 森下 和茂;小笹 史登;吉田 英樹;山口 政光
• 通讯作者: 山口 政光