Pathogenesis of intervertebral disc degeneration and functional analysis of vascular endothelial growth factor (VEGF) in the intervertebral disc

椎间盘退变的发病机制及椎间盘血管内皮生长因子（VEGF）的功能分析

• 批准号: 24791570
• 负责人: HIYAMA Akihiko
• 金额: $ 2.75万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24791570/
• 关键词: 脊椎脊髄病学; 椎間板変性; VEGF; Wntｼｸﾞﾅﾙ; 分子学的解析; 腰痛の解明

The goals of this study were to examine whether Wnt signaling accelerates vascular endotherial growth factor (VEGF) expression of nucleus pulposus cells. Rat nucleus pulposus cells were cultured under normoxic or hypoxic conditions, and the expression and promoter activity of Wnt signaling and VEGF were evaluated. Nucleus pulposus cells exhibited increased beta-catenin mRNA and protein under the hypoxic condition. Nucleus pulposus cells cotransfected with the WT-beta-catenin expression plasmid or Si-beta-catenin expression, or treated with a different concentration of BIO, showed a dose-dependent increase in the activity of VEGF (V5-luc). We next measured the relative expression level of VEGF mRNA after the treatment of BIO. We analyzed protein expression with western-blot. Total beta-catenin level after the treatment of BIO increased to a greater extent in nucleus pulposus cells compared with untreated cells.

本研究的目的是检查 Wnt 信号传导是否加速髓核细胞的血管内皮生长因子 (VEGF) 表达。在常氧或低氧条件下培养大鼠髓核细胞，评估Wnt信号和VEGF的表达和启动子活性。缺氧条件下，髓核细胞表现出β-连环蛋白mRNA和蛋白质增加。共转染WT-β-连环蛋白表达质粒或Si-β-连环蛋白表达，或用不同浓度的BIO处理的髓核细胞，显示VEGF（V5-luc）活性呈剂量依赖性增加。接下来我们测量了 BIO 处理后 VEGF mRNA 的相对表达水平。我们用蛋白质印迹分析了蛋白质表达。与未处理的细胞相比，BIO处理后髓核细胞中的总β-连环蛋白水平增加了更大程度。

项目成果

A complex interaction between Wnt signaling and TNF-α in nucleus pulposus cells.

• DOI: 10.1186/ar4379
• 发表时间: 2013-11-14
• 期刊: Arthritis research & therapy
• 影响因子: 4.9
• 作者: Hiyama A;Yokoyama K;Nukaga T;Sakai D;Mochida J
• 通讯作者: Mochida J

The effects of oxygen tension and antiaging factor Klotho on the Wnt singaling in nucleus pulposus cells.

氧张力和抗衰老因子 Klotho 对髓核细胞 Wnt 信号传导的影响。

• 发表时间: 2012
• 期刊: Arthritis Res Ther.
• 作者: Aini H;Ochi H;Iwata M;Okawa A;Koga D;Okazaki M;Sano A;Asou Y.;Akihiko Hiyama;Akihiko Hiyama
• 通讯作者: Akihiko Hiyama