Time-resolved spectroscopic characterization of blue-light photo-receptors

蓝光光感受器的时间分辨光谱表征

• 批准号: 5420251
• 负责人: Professor Dr. Bernhard Dick
• 金额: --
• 依托单位: Institut für Experimentelle und Angewandte Physik
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2004
• 资助国家: 德国
• 起止时间: 2003-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5420251?language=en
• 关键词: Time; resolved; spectroscopic; characterization; blue

We have analysed the structure and the photochemical processes of the LOV1-domain of the blue-light receptor Phot1 from Chlamydomonas. We propose to analyze the electron transfer process that has been assumed to occur in the triplet state of LOV1 (FMN-type) and to study the subsequent formation of the signaling states in Phot1.A highly interdisciplinary approach combining molecular biology, biochemistry, spectroscopy, crystallography, and theory will be undertaken. Second, we plan to study sensory blue light receptors containing the so-called BLUF domain as chromophores (FAD-type). We will express the AppA-Protein, which is involved in the regulation of genes for photosynthetic proteins in the purple bacterium R.sphaeroides, and PAC-a/b that function as light-regulated adenylate cyclases in the flagellate E.gracilis. We will characterize the light activated enzymatic activities and try to determine the structures of both proteins in the dark form, and the light-activated state. We will study the primary photoprocesses by time and wavelength resolved UV-Vis and fluorescence spectroscopy, in particular in the ns time range. The spectral and kinetic information will be combined with the structural data to describe in detail the physical and photochemical reactions involved in the formation of the signaling states.

我们分析了衣藻蓝光受体 Phot1 的 LOV1 结构域的结构和光化学过程。我们建议分析假设发生在 LOV1（FMN 型）三重态中的电子转移过程，并研究 Phot1 中信号状态的后续形成。这是一种结合了分子生物学、生物化学、光谱学、将进行晶体学和理论。其次，我们计划研究含有所谓 BLUF 结构域作为发色团（FAD 型）的感觉蓝光受体。我们将表达 AppA-Protein，它参与紫色细菌 R.sphaeroides 中光合蛋白基因的调节，以及 PAC-a/b，它在鞭毛虫 E.gracilis 中充当光调节腺苷酸环化酶。我们将表征光激活的酶活性，并尝试确定暗形式和光激活状态的两种蛋白质的结构。我们将通过时间和波长分辨的紫外-可见光和荧光光谱研究主要的光过程，特别是在纳秒时间范围内。光谱和动力学信息将与结构数据相结合，详细描述信号状态形成中涉及的物理和光化学反应。

项目成果

Microsecond light-induced proton transfer to flavin in the blue light sensor plant cryptochrome.

蓝光传感器植物隐花色素中微秒光诱导质子转移至黄素。

• DOI: 10.1021/ja901628y
• 发表时间: 2009-09-15
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Thomas Langenbacher;Dominik Immeln;B. Dick;T. Kottke
• 通讯作者: T. Kottke

Blue‐Light Induced Interaction of LOV Domains from Chlamydomonas reinhardtii

蓝光诱导莱茵衣藻 LOV 结构域的相互作用

• DOI: 10.1002/cbic.200800158
• 发表时间: 1938
• 期刊: ChemBioChem
• 影响因子: 3.2
• 作者: R. J. Kutta; E. S. A. Hofinger; H. Preuss; G. Bernhardt; B. Dick
• 通讯作者: B. Dick

Photoreaction of mutated LOV photoreceptor domains from Chlamydomonas reinhardtii with aliphatic mercaptans: implications for the mechanism of wild type LOV.

莱茵衣藻突变的 LOV 光感受器结构域与脂肪族硫醇的光反应：对野生型 LOV 机制的影响

• DOI: 10.1039/b922408d
• 发表时间: 2010
• 期刊: Physical chemistry chemical physics : PCCP
• 作者: K. Lanzl; M. v. S;en
• 通讯作者: en