Analysis of oocyte-specific gene, Oog1 and its involvement in molecular basis of zygotic gene activation

卵母细胞特异性基因 Oog1 分析及其参与合子基因激活的分子基础

• 批准号: 19380158
• 负责人: MINAMI Naojiro
• 金额: $ 10.57万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19380158/
• 关键词: 発生工学; Oog1; 胚性ゲノム; プロモーター活性; 卵母細胞; 胚性ゲノムの活性化; RNAi; 遺伝子発現; 初期発生; 0og1

In the present study, we investigated the function of oocyte-specific gene, Oog1. The gene translocates to the nucleus of fertilized embryos at the time of zygotic gene activation. In order to analyze the function of the gene, we produced the transgenic mice in which the mRNA of Oog1 is knockdown. In addition, we investigated the protein expression of Oog1 during female meiosis and it is revealed that the protein exists in the oocyte nucleus at the prophase of meiosis. In another experiments, we identified the regulatory regions of Oog1 which controls the expression of the gene.

在本研究中，我们研究了卵母细胞特异性基因 Oog1 的功能。在合子基因激活时，该基因易位至受精胚胎的细胞核。为了分析该基因的功能，我们制备了Oog1 mRNA被敲低的转基因小鼠。此外，我们研究了雌性减数分裂过程中Oog1蛋白的表达，发现该蛋白存在于减数分裂前期的卵母细胞核中。在另一项实验中，我们确定了 Oog1 控制基因表达的调控区域。

项目成果

Zygotic gene activation and maternal factors in mammals.

哺乳动物中的合子基因激活和母体因素。

• DOI: 10.1262/jrd.19029
• 发表时间: 2007-08-01
• 期刊: The Journal of reproduction and development
• 作者: N. Minami;Toru Suzuki;Satoshi Tsukamoto
• 通讯作者: Satoshi Tsukamoto

In vitro fertilization in inbred BALB/c mice: osmotic pressure and calcium are important factors for penetration ofperm through the zona pellucida.

近交系 BALB/c 小鼠的体外受精：渗透压和钙是精子穿过透明带的重要因素。

• 发表时间: 2008
• 作者: Kito; S; Ohta; Y
• 通讯作者: Y

Multipotential ability of primitive germ cells from neonatal pig testis cultured in vitro

体外培养新生猪睾丸原始生殖细胞的多能性

• 发表时间: 2009
• 作者: Goel; S
• 通讯作者: S

初期胚の発生と遺伝子

早期胚胎发育和基因

• 发表时间: 2009
• 作者: 南直治郎

Reprogramming events of mammalian somatic cells induced by Xenopus laevis egg extracts.

非洲爪蟾卵提取物诱导的哺乳动物体细胞重编程事件。

• 发表时间: 2007
• 作者: Miyamoto; K.
• 通讯作者: K.