Feasibility of implantable liver tissues having a vascular-like blood flow network

具有血管样血流网络的植入性肝组织的可行性

• 批准号: 19360371
• 负责人: SAKAI Yasuyuki
• 金额: $ 10.48万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19360371/
• 关键词: 肝組織工学; 胎児肝細胞; 移植; 三次元担体; 液性因子; 酸素供給; 血流導入; 生物・生体工学; ES細胞

Combination of 3D culture and soluble factors was very effective in maturation of fetal rat hepatocytes in vitro. Oxygen supply at appropriate exposure concentrations was also important to fully utilize the self-organization capacity of such hepatocyte progenitor population. Control of blood cell-to-implanted hepatocytes was shown to be a crucial issue in designing and engineering of implantable pre-vascularized liver tissues.

3D 培养物与可溶性因子的结合对于胎鼠肝细胞的体外成熟非常有效。适当暴露浓度的氧气供应对于充分利用此类肝细胞祖细胞群的自组织能力也很重要。血细胞与植入肝细胞的控制被证明是可植入预血管化肝组织设计和工程中的一个关键问题。

项目成果

Transplantation of a fetal liver cell-loaded hyaluronic acid sponge onto the mesentery recovers a Wilson's disease model rat.

将载有胎儿肝细胞的透明质酸海绵移植到肠系膜上可恢复威尔逊病模型大鼠。

• DOI: 10.1093/jb/mvq063
• 发表时间: 2010-09-01
• 期刊: Journal of biochemistry
• 作者: T. Katsuda;T. Teratani;T. Ochiya;Y. Sakai
• 通讯作者: Y. Sakai

Soluble factor-dependent in vitro growth and maturation of rat fetal liver cells in a three-dimensional culture system

三维培养系统中大鼠胎肝细胞可溶性因子依赖性体外生长和成熟

• 发表时间: 2008
• 作者: S.Hanada; N.Kojima; Y.Sakai
• 通讯作者: Y.Sakai

胚性幹細胞を用いた肝細胞分化誘導研究の現状

利用胚胎干细胞诱导肝细胞分化的研究现状

• 发表时间: 2009
• 作者: 勝田毅; 小森喜久夫; 酒井康行
• 通讯作者: 酒井康行

Direct oxygen supply with poly dimethylsiloxane (PDMS) membranes induces a spontaneous organization of thick heterogeneous liver tissues from rat fetal liver cells in vitro

聚二甲基硅氧烷（PDMS）膜直接供氧诱导大鼠胎儿肝细胞自发组织厚的异质肝组织

• 作者: M. Hamon; S. Hanada; T. Fujii; Y. Sakai
• 通讯作者: Y. Sakai

Contributions of Nano- and Micro-Technologies to Liver Tissue Engineering, 招待講演

纳米和微米技术对肝脏组织工程的贡献，特邀报告

• 发表时间: 2008
• 作者: Y. Sakai