Effects of the low - level laser irradiation on arthritis

低强度激光照射对关节炎的影响

基本信息

批准号：
18592146
负责人：
OHTA Mitsuhiro
金额：
$ 2.21万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

The purpose of this research is to demonstrate the effect for low-level laser irradiation over the living body proving the experiment system using an animal. The pathologic model of rheumatoid arthritis was product and the effect for inflammation by low-level laser irradiation was examined by performing diode laser irradiation and FEL irradiation.We use rat as laboratory animals. The rat received a subcutaneous injection of type II collagen of the cow as an antigen to back, and the symptoms of arthritis were induced. The animal was divided into three groups as five per group, the 1st group was the control group, the 2nd group was the CIA group performing collagen sensitization which it has not laser irradiated, and the 3rd group was considered as the CIA+LLI group which performed collagen sensitization and also carried out laser irradiation every several days. The general state of the rat was observed during the experimental periods, it measured weight once per week, measured swelling of the joint portion with slide calipers, and extracted blood from the jugular vein.As a result, it was accepted that two kinds of low-level laser irradiation controls swelling of the hind foot of the rat which was caused experimentally in any case. Moreover, when the concentration in the serum of IL-1beta and IL-6 which is inflammatory cytokine were measured, as compared with the CIA group, it was falling intentionally by the CIA+LLI group. From these results, it was suggested that low-level laser irradiation has an effect which controls swelling by inflammation. Furthermore, it was suggested that low-level laser irradiation is a cure which can expect the noninvasive anti-inflammation effect without side effects.

本研究的目的是通过动物实验系统来证明低强度激光照射对活体的影响。制作类风湿性关节炎的病理模型，并通过二极管激光照射和FEL照射来考察低强度激光照射对炎症的影响。我们以大鼠为实验动物。大鼠皮下注射牛II型胶原蛋白作为抗原背部，诱发关节炎症状。将动物分为三组，每组5只，第1组为对照组，第2组为未进行激光照射的胶原致敏CIA组，第3组为CIA+LLI组，未进行激光照射。进行胶原蛋白致敏，并每隔几天进行一次激光照射。实验期间观察大鼠的一般状态，每周称一次体重，用游标卡尺测量关节部肿胀情况，并从颈静脉抽血。结果认为，两种低水平激光照射控制了在任何情况下实验引起的大鼠后足的肿胀。另外，测定血清中作为炎性细胞因子的IL-1β和IL-6的浓度时，与CIA组相比，CIA+LLI组有意降低。由这些结果表明，低强度激光照射具有抑制炎症引起的肿胀的效果。此外，有人提出，低强度激光照射是一种可以预期无创抗炎效果且无副作用的治疗方法。