The roles and the kinetics of gp91 phox after traumatic brain injury in mice

gp91 phox在小鼠脑外伤后的作用和动力学

• 批准号: 18591989
• 负责人: DOHI Kenji
• 金额: $ 2.36万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591989/
• 关键词: free radical; neurotrauma; oxidative stress; animal model; mice; knockout; gp91phox; NADPH; 神経科学; 頭部外傷; ラット; ESR; gp91

(Introduction)The sources of superoxide radical (O2-) production following traumatic brain injury (TBI) are known as some cascades: cyclooxygenase, xanthine oxygenase, NADPH oxygenase. In this study, the kinetics and the roles of gp9lphox(gp91) after traumatic brain injury were investigated using gp9lphox gene knockout mouse.(Material and Methods)C57BL mice were used in this study. In the current study, we investigated the time-dependent changes in the expression of the gp9lphox in the mice traumatic brain injury model (TBI) (controlled cortical impact model) using western blotting method. The gp91 positive cells were identified by double immunohistochemical technique. The damaged areas of wild mouse and gp9lphox knockout mouse were also measured on day2 after TBI. Superoxide radical levels were detected by in situ detection of oxidized Het.(Results)On day 2 after TBI, the gp91 phox was strongly expressed in microglia mainly. The damaged area was suppressed in gp91 phox knockout mouse. Production of superoxide radical was also suppressed in gp91 phox knockout mouse.These results indicate that gp91phox and NADPH has the important roles as a generator of free radical in TBI.

（简介）创伤性脑损伤 (TBI) 后超氧自由基 (O2-) 产生的来源被称为级联：环氧合酶、黄嘌呤加氧酶、NADPH 加氧酶。本研究利用gp9lphox基因敲除小鼠研究了gp9lphox(gp91)在脑外伤后的动力学和作用。(材料与方法)本研究使用C57BL小鼠。在本研究中，我们利用蛋白质印迹法研究了小鼠创伤性脑损伤模型（TBI）（受控皮质冲击模型）中 gp9lphox 表达的时间依赖性变化。采用双重免疫组化技术鉴定gp91阳性细胞。 TBI后第2天还测量了野生小鼠和gp9lphox敲除小鼠的受损面积。通过原位检测氧化Het来检测超氧自由基水平。(结果)TBI后第2天，gp91 phox主要在小胶质细胞中强表达。 gp91 phox 敲除小鼠中受损区域受到抑制。 gp91 phox 敲除小鼠中超氧自由基的产生也受到抑制。这些结果表明，gp91phox 和 NADPH 作为自由基产生剂在 TBI 中具有重要作用。

项目成果

New Frontiers in Regenerative Medicine(The Surgical Procedures of Hippocampal Ischemia Models for the Study of Regeneration in Rats)

再生医学新前沿（用于研究大鼠再生的海马缺血模型的手术方法）

• 发表时间: 2007
• 作者: Dohi K; Ohtaki H; Kudo Y; Nakamachi T; Shioda S; Aruga T
• 通讯作者: Aruga T

Increased mitochondrial DNA oxidative damage after transient middle cerebral artery occlusion in mice

小鼠短暂大脑中动脉闭塞后线粒体 DNA 氧化损伤增加

• 发表时间: 2007
• 作者: Ohtaki; H; Takeda; T; Dohi; K; Yofu; S; Nakamachi; T; Satoh; K; Hiraizumi; Y; Miyaoka; H; Matsunaga; M; Shioda; S
• 通讯作者: S

Pituitary adenylate cyclase-activating polypeptide (PACAP) decreases ischemic neuronal cell death in association with IL-6.

垂体腺苷酸环化酶激活多肽 (PACAP) 可减少与 IL-6 相关的缺血性神经元细胞死亡。

• DOI: 10.1073/pnas.0600375103
• 发表时间: 2006-05-09
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: H. Ohtaki;T. Nakamachi;K. Dohi;Y. Aizawa;A. Takaki;K. Hodoyama;S. Yofu;H. Hashimoto;N. Shintani;A. Baba;M. Kopf;Y. Iwakura;K. Matsuda;A. Arimura;S. Shioda
• 通讯作者: S. Shioda

Does edaravone(MCI-186) act as an antioxidant and a neuroprotector in experimental traumatic brain injury?

依达拉奉 (MCI-186) 在实验性脑外伤中充当抗氧化剂和神经保护剂吗？

• 发表时间: 2007
• 作者: Dohi K; Satoh K; Yofu S; Yoshikawa T; Shioda S; Aruga T.
• 通讯作者: Aruga T.

The roles of gp91phox in Traumatic brain injury in mice(Best presentation Awards)

gp91phox在小鼠创伤性脑损伤中的作用（最佳演讲奖）

• 发表时间: 2007
• 作者: Kenii; Dohi; Tomoya; Nakamachi; Sachiko; Yofu; Yuko; Mihara; Kentaro; Morikawa; Hirokazu; Ohtaki; Kazue; Satoh; Seiji; Shioda; Tohru; Aruga
• 通讯作者: Aruga