Bone and vascular signals during the repair process after bone and bone marrow injury

骨和骨髓损伤后修复过程中的骨和血管信号

• 批准号: 18390422
• 负责人: SAKAI Akinori
• 金额: $ 5.12万
• 依托单位: University of Occupational and Environmental Health, Japan
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390422/
• 关键词: 骨髄損傷; 骨芽細胞; シグナル伝達; ビタミンA; 血管; VEGF; PECAM-1; BMP-2; HIF; HIF-1; 骨・骨髄損傷; HIF-1α; オステオカルシン; 骨形成; 血管形成; 髄内仮骨; flt-1; 破骨細胞; 骨吸収; 代謝回転; 骨量

The dis ruption of tyrosine kinase domain of flt-1, one of VEGF (vascular endothelial growth factor) receptors, disturbed osteoblast differentia tion and delayed the repair process after bone and bone marrow injury in mice. The production of alkaline phosphatase increased in osteoblasts co-cultured with endothelial cells. This increase was dose-dependently inhibited by PECAM-1 (platelet endothelial cell adhesion molecule-1) antibody. These findings demonstrated that vascular factors including VEGF and PECAM-1 significantly contributed to osteoblast development in the repairprocess afterbone and bone marrow injury.

flt-1（VEGF（血管内皮生长因子）受体之一）酪氨酸激酶结构域的破坏会扰乱成骨细胞的分化，并延迟小鼠骨和骨髓损伤后的修复过程。与内皮细胞共培养的成骨细胞中碱性磷酸酶的产生增加。这种增加受到 PECAM-1（血小板内皮细胞粘附分子-1）抗体的剂量依赖性抑制。这些发现表明，包括 VEGF 和 PECAM-1 在内的血管因子在骨和骨髓损伤修复过程中显着促进成骨细胞发育。

项目成果

Genetic disruption of all nitric oxide synthase isoforms enhances bone tournover and bone mineral density in mice in vivo

所有一氧化氮合酶亚型的遗传破坏可增强小鼠体内的骨周转和骨矿物质密度

• 发表时间: 2006
• 作者: Sabanai K; Sakai A; Nakamura T; et.al
• 通讯作者: et.al

Genetic disruption of all NO synthase isoforms enhances BMD and bone turnover in mice in vivo: involvement of the renin-angiotensin system.

所有NO合酶亚型的基因破坏可增强小鼠体内的BMD和骨转换：肾素-血管紧张素系统的参与。

• 发表时间: 2008
• 作者: Sabanai K; Tsutsui M; Sakai A; Hirasawa H; Tanaka S; Nakamura E; Tanimoto A; Sasaguri Y; Ito M; Shimokawa H; Nakamura T; Yanagihara N
• 通讯作者: Yanagihara N

1章骨構造と機能細胞とその機能、最新整形外科学大系1運動器の生物学と生体力学(総編集越智隆弘、専門編集中村利孝、吉川秀樹)

第一章骨结构和功能细胞及其功能，最新骨科科学1运动器官的生物学和生物力学（总编辑Takahiro Ochi，专业编辑Toshitaka Nakamura，Hideki Yoshikawa）

• 发表时间: 2008
• 作者: 酒井昭典

Cyclooxygenase-2 selective inhibition suppresses restoration of tibial trabecular bone formation in association with restriction of osteoblast maturation in skeletal reloading after hindlimb elevation of mice.

环氧合酶 2 选择性抑制抑制胫骨小梁骨形成的恢复，与小鼠后肢抬高后骨骼重新加载中成骨细胞成熟的限制相关。

• DOI: 10.1016/j.bone.2005.12.014
• 发表时间: 2006-07-01
• 期刊: Bone
• 影响因子: 4.1
• 作者: Kenichiro Nakai;Shinya Tanaka;A. Sakai;M. Nagashima;Masahiro Tanaka;H. Otomo;Toshitaka Nakamura
• 通讯作者: Toshitaka Nakamura

Flt-1 tyrosine kinase-deficient homozygous mice result in decreased trabecular bone volume with reduced osteogenic potential

Flt-1酪氨酸激酶缺陷纯合小鼠导致小梁骨体积减少，成骨潜力降低

• 发表时间: 2007
• 作者: Otomo H; Sakai A; et. al.
• 通讯作者: et. al.